Rapid DNA library construction for functional genomic and metagenomic screening Journal Article
| Authors: | Schmitz, J. E.; Daniel, A.; Collin, M.; Schuch, R.; Fischetti, V. A. |
| Article Title: | Rapid DNA library construction for functional genomic and metagenomic screening |
| Abstract: | A rapid protocol was developed for constructing plasmid libraries from small quantities of genomic/metagenomic DNA. The technique utilizes linker amplification with topoisomerase cloning and allows for inducible transcription in Escherichia coli. As proof of principle, several anti-Bacillus lysins were cloned from bacteriophage genomes and an aerolysin was cloned from a metagenomic sample. Copyright © 2008, American Society for Microbiology. All Rights Reserved. |
| Keywords: | nonhuman; gene amplification; gene expression; transcription, genetic; gene library; molecular cloning; cloning, molecular; genome analysis; dna; nucleotide sequence; escherichia coli; molecular analysis; plasmid; genomics; plasmids; genome; coliform bacterium; bacterial genome; microbiology; genetic screening; genomic dna; lysine; dna topoisomerase; enzyme; clone; cloning; bacillus; bacteriophage; bacterial toxins; dna library; toxin; bacteriophages; aerolysin; metagenomic screening; plasmid libraries; nucleic acid amplification techniques; pore forming cytotoxic proteins |
| Journal Title: | Applied and Environmental Microbiology |
| Volume: | 74 |
| Issue: | 5 |
| ISSN: | 0099-2240 |
| Publisher: | American Society for Microbiology |
| Date Published: | 2008-03-01 |
| Start Page: | 1649 |
| End Page: | 1652 |
| Language: | English |
| DOI: | 10.1128/aem.01864-07 |
| PUBMED: | 18083885 |
| PROVIDER: | scopus |
| PMCID: | PMC2258639 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 7" - "Export Date: 17 November 2011" - "CODEN: AEMID" - "Molecular Sequence Numbers: GENBANK: AAC44637, AF064048, AF41110466, AY157998, AY378303, AY611033, EU258891, EU258892, EU258894, M16495, M84709, X65044, X65045, X65046, X65048, Y00559;" - "Source: Scopus" |
