Herpes oncolytic therapy of salivary gland carcinomas Journal Article

Authors: Reid, V.; Yu, Z.; Schuman, T.; Li, S.; Singh, P.; Fong, Y.; Wong, R. J.
Article Title: Herpes oncolytic therapy of salivary gland carcinomas
Abstract: Oncolytic herpes simplex viruses (HSV) have demonstrated potent antitumoral effects against a variety of human malignancies in preclinical studies and are in early clinical trials. We explored the activity of an attenuated, replication-competent, oncolytic HSV (NV1023) for the treatment of human salivary gland carcinomas. NV1023 was able to successfully enter into 4 mucoepidermoid carcinoma (H292, H3118, HTB-41, UT-MUC-1) and 2 adenocarcinoma (HSY, HSG) cell lines, as measured by lacZ assays after exposure to 5 viral particles per cell (MOI 5). Viral plaque assays showed variation of viral replication within these cell lines, ranging from a 268-fold increase (H292) to a 3-fold increase (HSG) in viral titer. At MOI 5, all cell lines showed >95% cytotoxicity from NV1023 by Day 7, except for HSY (73%). At MOI 0.1, H3118 and UT-MUC-1 remained highly sensitive to NV1023, both showing >95% cytotoxicity by Day 7. The mucoepidermoid carcinomas were more sensitive to NV1023 at low viral concentrations compared with the adenocarcinomas. Flank tumors of H3118, HTB-41 and HSY in nude mice showed significant tumor volume reductions after a single intratumoral injection of NV1023 (2 × 107 plaque-forming units). These data suggest that oncolytic herpes viruses have significant efficacy entering, replicating within, and lysing human salivary gland carcinomas. These promising biologic agents should be further investigated as novel therapy for patients with salivary carcinomas failing conventional treatment. © 2007 Wiley-Liss, Inc.
Keywords: controlled study; unclassified drug; human cell; nonhuman; cell proliferation; mouse; animals; mice; animal tissue; cell survival; animal experiment; animal model; cytotoxicity; tumor cells, cultured; mice, nude; oncolytic herpes virus; simplexvirus; oncolytic virotherapy; salivary gland; carcinoma, mucoepidermoid; salivary gland neoplasms; virus replication; herpes; virus particle; adenocarcinoma, follicular; salivary gland carcinoma; nv 1023; herpesviridae infections; oncolysis; viral
Journal Title: International Journal of Cancer
Volume: 122
Issue: 1
ISSN: 0020-7136
Publisher: John Wiley & Sons  
Date Published: 2008-01-01
Start Page: 202
End Page: 208
Language: English
DOI: 10.1002/ijc.23030
PUBMED: 17764117
PROVIDER: scopus
Notes: --- - "Cited By (since 1996): 5" - "Export Date: 17 November 2011" - "CODEN: IJCNA" - "Source: Scopus"
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MSK Authors
  1. Zhenkun Yu
    24 Yu
  2. Paramjeet Singh
    4 Singh
  3. Vincent J Reid
    8 Reid
  4. Richard J Wong
    228 Wong
  5. Yuman Fong
    741 Fong
  6. Sen Li
    19 Li