Sensitivity of squamous cell carcinoma lymph node metastases to herpes oncolytic therapy Journal Article
| Authors: | Yu, Z.; Li, S.; Huang, Y. Y.; Lin, S. F.; Fong, Y.; Wong, R. J. |
| Article Title: | Sensitivity of squamous cell carcinoma lymph node metastases to herpes oncolytic therapy |
| Abstract: | Purpose: Cancer metastases may have phenotypic and genetic differences from their primary cancers of origin. Engineered, replication-competent, attenuated viruses based on herpes simplex virus-1 (HSV-1) have shown potent oncolytic effects in treating primary tumors in animal tumor models, but their efficacy in treating lymph node metastases is poorly understood. We compared the efficacy of an attenuated oncolytic HSV-1 (NV1023) in treating a series of murine squamous carcinoma cell lines derived from serial implantation and harvest from metastatic lymph nodes. Experimental Design and Results: The auricles of C3H/HeJ mice were implanted with SCCVII. Cervical nodal metastases were isolated, expanded in vitro, and reimplanted into new mice. A series of cell lines (LN1-LN7) were generated through seven serial passages. Cells from higher LN passages showed consistent trends toward increased migratory and invasive ability, increased cell surface nectin-1 (an HSV-1 receptor) expression, and increased glycoprotein D binding. Exposure to NV1023 showed increased viral entry, replication, and cytotoxicity with higher LN passages. Intratumoral injection of NV1023 in a murine flank tumor model caused significantly greater tumor regression and increased viral infection of LN7 compared with SCCVII. Conclusions: These results show that lymph node metastases may undergo selection for characteristics, including increased nectin-1 expression, that make them more sensitive targets for herpes oncolytic therapy. These findings support the clinical application of these agents for the treatment of lymph node metastases. ©2008 American Association for Cancer Research. |
| Keywords: | controlled study; unclassified drug; squamous cell carcinoma; carcinoma, squamous cell; nonhuman; lymph node metastasis; lymphatic metastasis; animal cell; mouse; animals; mice; gene expression; skin neoplasms; animal experiment; animal model; protein binding; herpes simplex; cancer cell culture; cytotoxicity; in vitro study; tumor regression; cell line, tumor; virus receptor; cancer invasion; disease model; cancer vaccine; gene therapy; oncolytic viruses; oncolytic virotherapy; cell migration; cell adhesion molecules; herpesvirus 1, human; neoplasm invasiveness; virus replication; cell surface protein; nv 1023; mice, inbred c3h; cho cells; cricetinae; cricetulus; virus entry; viral envelope proteins; glycoprotein d; herpes simplex virus 1 receptor; nectin 1; virus internalization |
| Journal Title: | Clinical Cancer Research |
| Volume: | 14 |
| Issue: | 6 |
| ISSN: | 1078-0432 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 2008-03-15 |
| Start Page: | 1897 |
| End Page: | 1904 |
| Language: | English |
| DOI: | 10.1158/1078-0432.ccr-07-4615 |
| PUBMED: | 18347193 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 2" - "Export Date: 17 November 2011" - "CODEN: CCREF" - "Source: Scopus" |
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