Generation of temperature sensitive mutations with error-prone PCR in a gene encoding a component of the spindle pole body in fission yeast Journal Article
| Authors: | Tang, N. H.; Fong, C. S.; Masuda, H.; Jourdain, I.; Yukawa, M.; Toda, T. |
| Article Title: | Generation of temperature sensitive mutations with error-prone PCR in a gene encoding a component of the spindle pole body in fission yeast |
| Abstract: | Temperature-sensitive (ts) mutants provide powerful tools for investigation of cellular functions of essential genes. We report here asimple procedure to generate ts mutations using error-prone PCR within pcp1 that encodes aspindle pole body (SPB) component in Schizosaccharomyces pombe. This manipulation is not restricted to pcp1, and can be suited to any essential genes involved in other processes. © 2019 Informa UK Limited, trading as Taylor & Francis Group. |
| Keywords: | yeast; gene encoding; fission yeast; errors; encoding (symbols); signal encoding; error-prone pcr; pcp1; temperature sensitive mutants; the spindle pole body; error prone pcr; spindle pole bodies; temperature sensitive; poles |
| Journal Title: | Bioscience, Biotechnology and Biochemistry |
| Volume: | 83 |
| Issue: | 9 |
| ISSN: | 0916-8451 |
| Publisher: | Taylor & Francis |
| Date Published: | 2019-09-01 |
| Start Page: | 1717 |
| End Page: | 1720 |
| Language: | English |
| DOI: | 10.1080/09168451.2019.1611414 |
| PUBMED: | 31042107 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
