A temperature-sensitive mutation of the vaccinia virus E11 gene encoding a 15-kDa virion component Journal Article
| Authors: | Wang, S. P.; Shuman, S. |
| Article Title: | A temperature-sensitive mutation of the vaccinia virus E11 gene encoding a 15-kDa virion component |
| Abstract: | The ts49 mutation of vaccinia virus WR was mapped by marker rescue to the E11 gene encoding a 15-kDa polypeptide. During synchronous infection of BSC40 cells with wild-type virus, immunoreactive E11 protein accumulated in parallel with the onset of late protein synthesis. Immunoblotting of extracts of wild-type virions showed that the E11 protein was encapsidated within the virus core. A normal temporal pattern of viral protein synthesis was observed in cells infected with ts49 at the nonpermissive temperature (40°) and normal-appearing ts49 progeny virions were observed by electron microscopy. Sequencing of the E11 gene of ts49 revealed a single amino acid substitution, Gly(66)Arg, in the mutant E11 polypeptide. The steady-state level of E11 protein during ts49 infection was much lower than that observed during infection with wild-type virus. This was the case at both the permissive and nonpermissive temperatures. We discuss two possible explanations for the thermosensitive growth of ts49: (i) that virus infectivity requires a threshold level of active E11 protein or (ii) that E11 function is conditionally essential. |
| Keywords: | controlled study; nonhuman; animal cell; animalia; protein synthesis; vaccinia virus; virus replication; virus protein; vaccinia; virus infectivity; temperature sensitive mutant; priority journal; article |
| Journal Title: | Virology |
| Volume: | 216 |
| Issue: | 1 |
| ISSN: | 0042-6822 |
| Publisher: | Elsevier Inc. |
| Date Published: | 1996-02-01 |
| Start Page: | 252 |
| End Page: | 257 |
| Language: | English |
| DOI: | 10.1006/viro.1996.0057 |
| PUBMED: | 8614998 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
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