Secretogranin I/chromogranin B Is a heparin‐binding adhesive protein Journal Article
| Authors: | Chen, M.; Tempst, P.; Yankner, B. A. |
| Article Title: | Secretogranin I/chromogranin B Is a heparin‐binding adhesive protein |
| Abstract: | Abstract: The major heparin‐binding protein secreted by PC12 cells was purified from conditioned medium. Amino‐terminal sequencing of the purified protein identified it as secretogranin I/chromogranin B (SgI/ChmB). The protein showed the same electrophoretic mobility and biochemical characteristics as previously reported for SgI/ChmB and could be purified in high yield using a simple procedure. In vitro experiments demonstrated that SgI/ChmB effectively promoted cell‐substratum adhesion of NIH 3T3 and PC12 cells and supported neurite outgrowth in primary hippocampal neurons. Thus, SgI/ChmB may be a new member of the family of heparin‐binding extracellular matrix proteins that mediate cell adhesion and support neurite outgrowth. Copyright © 1992, Wiley Blackwell. All rights reserved |
| Keywords: | nonhuman; proteins; animal cell; animal; tumor cells, cultured; animalia; extracellular matrix; amino acid sequence; molecular sequence data; amino terminal sequence; rat; heparin; biochemistry; cell adhesion; electrophoretic mobility; hippocampus; neurite outgrowth; nerve fiber growth; neurites; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; heparin binding protein; chromogranin b; secretogranin i |
| Journal Title: | Journal of Neurochemistry |
| Volume: | 58 |
| Issue: | 5 |
| ISSN: | 0022-3042 |
| Publisher: | Wiley Blackwell |
| Date Published: | 1992-05-01 |
| Start Page: | 1691 |
| End Page: | 1698 |
| Language: | English |
| DOI: | 10.1111/j.1471-4159.1992.tb10042.x |
| PUBMED: | 1560226 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Source: Scopus |
