Analysis of BCL2 and MYC expression in non-hodgkin’s lymphomas by in situ hybridization: Correlation with chromosome translocations Journal Article
| Authors: | Murty, V. V. V. S.; Ladanyi, M.; Houldsworth, J.; Mikraki, V.; Chaganti, R. S. K. |
| Article Title: | Analysis of BCL2 and MYC expression in non-hodgkin’s lymphomas by in situ hybridization: Correlation with chromosome translocations |
| Abstract: | We have used an in situ hybridization method for analysis of expression of BCL2 and MYC on cytospun preparations of normal and malignant lymphoid cell lines and tissue sections of normal and malignant lymph nodes. The probes comprised 50-mer antisense oligonucleotides starting at the ATG codons of exon 3 of BCL2 and exon 2 of MYC. We studied the expression of these two genes in frozen tissue sections of biopsy specimens derived from normal and hyperplastic lymph nodes, B-cell lymphomas carrying the t(14;18)(q32;q21) and t(8;14)(q24;q32) translocations, and T-cell lymphomas with clonal chromosome abnormalities. While all proliferating cells expressed both genes, BCL2 expression was increased two- to threefold in follicular lymphomas with t(14;18) and MYC expression was increased two- to fourfold in high-grade lymphomas with t(8;14). These results are consistent with previous data on deregulated expression of these genes obtained from study of lymphoma cell lines carrying the relevant translocations. © Lippincott-Raven Publishers. |
| Keywords: | oncoprotein; genetics; proto-oncogene proteins; molecular genetics; metabolism; proto oncogene; gene expression; pathology; tumor cells, cultured; chromosomes, human, pair 8; in situ hybridization; nonhodgkin lymphoma; dna; gene rearrangement; molecular sequence data; cell culture; messenger rna; lymphoma, non-hodgkin; rna, messenger; nucleotide sequence; bcl2; genes, myc; dna, neoplasm; chromosome translocation; base sequence; chromosome 8; lymphoid tissue; chromosome 18; proto-oncogene proteins c-bcl-2; oncogene myc; myc; proto-oncogenes; chromosomes, human, pair 14; chromosomes, human, pair 18; chromosome 14; oligonucleotide probe; oligonucleotide probes; human; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; chromosome translocations; translocation (genetics); proto oncogene proteins; proto oncogene proteins c bcl 2; non-hodgkin’s lymphoma |
| Journal Title: | Diagnostic Molecular Pathology |
| Volume: | 1 |
| Issue: | 4 |
| ISSN: | 1052-9551 |
| Publisher: | Lippincott Williams & Wilkins |
| Date Published: | 1992-12-01 |
| Start Page: | 221 |
| End Page: | 228 |
| Language: | English |
| PUBMED: | 1342969 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 30 July 2019 -- Source: Scopus |
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