Cell proliferation in rat colon measured with bromodeoxyuridine, proliferating cell nuclear antigen, and [(3)H]thymidine Journal Article
| Authors: | Richter, F.; Richter, A.; Yang, K.; Lipkin, M. |
| Article Title: | Cell proliferation in rat colon measured with bromodeoxyuridine, proliferating cell nuclear antigen, and [(3)H]thymidine |
| Abstract: | Epithelial cell proliferation was studied in the normal colonic mucosa of 5-week-old Sprague-Dawley rats, comparing [3H]thymidine incorporation (group 1) with two newer proliferation markers, bromodeoxyuridine (group 2) and proliferating cell nuclear antigen (group 3). Microautoradiography (group 1) or immunoperoxidase assays (groups 2 and 3) were carried out. Cells were counted for positive reaction and position along 50 colonic crypt columns/animal. No significant differences were found in number or distribution of labeled epithelial cells in proliferative compartments in crypt columns of normal colonic mucosa; labeled cells were mainly in the lower 60% of colonic crypts. Thus, in this model, bromodeoxyuridine and proliferating cell nuclear antigens were comparable to [3H]thymidine as reliable markers of proliferating epithelial cells in rat colon. © 1992, American Association for Cancer Research. All rights reserved. |
| Keywords: | immunohistochemistry; comparative study; biological marker; biological markers; animal; cytology; cell division; nuclear protein; nuclear proteins; diagnostic agent; rat; epithelium cell; epithelial cells; autoantigen; autoantigens; cell count; rats; cycline; rats, sprague-dawley; evaluation studies; thymidine; broxuridine; bromodeoxyuridine; intestine mucosa; tritium; proliferating cell nuclear antigen; intestinal mucosa; rat strain; female; article; support, non-u.s. gov't; fixatives; fixative |
| Journal Title: | Cancer Epidemiology Biomarkers and Prevention |
| Volume: | 1 |
| Issue: | 7 |
| ISSN: | 1055-9965 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 1992-11-01 |
| Start Page: | 561 |
| End Page: | 566 |
| Language: | English |
| PUBMED: | 1363835 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 30 July 2019 -- Source: Scopus |
