Specific and sensitive high-performance liquid chromatographic method with fluorescence detection for measurement of lometrexol and Its polyglutamates in biologic samples Journal Article
| Authors: | Muindi, J. R. F.; Young, C. W.; Shih, C. |
| Article Title: | Specific and sensitive high-performance liquid chromatographic method with fluorescence detection for measurement of lometrexol and Its polyglutamates in biologic samples |
| Abstract: | A reversed-phase high-performance liquid chromatographic (HPLC) assay is described for the quantitative determination of lometrexol in biological samples; the assay is rapid, simple, specific, and highly sensitive. The method requires the dissociation of lometrexol from folate-binding proteins present in blood and formation of a fluorescent oxidized derivative of the compound. The dissociation of lometrexol from folate-binding proteins was achieved by acidification to pH 3.5 using ammonium formate, followed by serum protein precipitation with perchloric acid. The protein-free lometrexol was subsequently oxidized by MnO2 at 90 degrees C for 10 min. Chromatographic separation of lometrexol without interference was achieved on a C-18 reversed-phase column with a convex gradient, using acetonitrile-0.1% ammonium formate, pH 7.0, as the mobile phase. In human serum and urine the calibration curve was linear between 5 and 300 nM. The lower limit of quantification was 5 nM. The method has been applied successfully to measure serum and urinary levels of lometrexol in patients. |
| Keywords: | 5,10-dideaza-5,6,7,8-tetrahydrofolic acid |
| Journal Title: | Journal of Chromatography. Biomedical Applications |
| Volume: | 621 |
| Issue: | 1 |
| ISSN: | 0378-4347 |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 1993-11-17 |
| Start Page: | 55 |
| End Page: | 64 |
| Language: | English |
| ACCESSION: | WOS:A1993MP71900008 |
| DOI: | 10.1016/0378-4347(93)80076-g |
| PROVIDER: | wos |
| PUBMED: | 7508451 |
| Notes: | Article -- Source: Wos |
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