Alteration in cholesteatoma fibroblasts: Induction of neoplastic-like phenotype Conference Paper
| Authors: | Parisier, S. C.; Agresti, C. J.; Schwartz, G. K.; Han, J. C.; Albino, A. P. |
| Title: | Alteration in cholesteatoma fibroblasts: Induction of neoplastic-like phenotype |
| Conference Title: | 27th Annual Meeting of the American Neurotology Society |
| Abstract: | Cholesteatomas are invasive, locally destructive skin-like lesions that, after operative removal, have a significant recidivistic rate. These aggressive characteristics suggest a fundamental alteration in the biology of one or more of its constituents (i.e., keratinocytes, fibroblasts, and inflammatory cells). The cholesteatoma matrix was studied by subculturing it into its main cellular components. Short-term cell cultures of fibroblasts from cholesteatoma matrix and controls from ear canal and postauricular skin were established. These cultured fibroblasts were tested for invasiveness using the Boyden Chamber Assay of Albini. The cells were evaluated for their ability to migrate, attach to, and invade a basement membrane. Results of the motility and attachment of fibroblasts cultured from cholesteatoma, canal wall, and postauricular skin did not differ. However, fibroblasts from 12 of 12 cholesteatoma specimens were highly invasive, while those from postauricular and ear canal skin were either weakly invasive or not invasive. This represents the first report of an intrinsic defect in the biology of cholesteatoma. Invasive fibroblasts that demonstrate a loss of growth control, may provide a contact guidance mechanism that aggressively mobilizes the squamous keratinizing epithelium contributing to the invasive, aggressive behavior of cholesteatoma. |
| Keywords: | tumor-cells; bone-resorption; middle-ear cholesteatoma |
| Journal Title | American Journal of Otology |
| Volume: | 14 |
| Issue: | 2 |
| Conference Dates: | 1992 Apr 11-12 |
| Conference Location: | Palm Desert, CA |
| ISBN: | 0192-9763 |
| Publisher: | Lippincott Williams & Wilkins |
| Date Published: | 1993-03-01 |
| Start Page: | 126 |
| End Page: | 130 |
| Language: | English |
| ACCESSION: | WOS:A1993KQ08400006 |
| PROVIDER: | wos |
| PUBMED: | 8503484 |
| Notes: | The pdf states that the paper was presented at the 125th Annual Meeting of the American Otological Society, but the dates given are for those of the Neurotology Society meeting listed above; the latter meeting took place Apr 13-14 at the same location -- Article -- Source: Wos |
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