Assessment of urinary 3-indoxyl sulfate as a marker for gut microbiota diversity and abundance of Clostridiales Journal Article
| Authors: | Farowski, F.; Els, G.; Tsakmaklis, A.; Higgins, P. G.; Kahlert, C. R.; Stein-Thoeringer, C. K.; Bobardt, J. S.; Dettmer-Wilde, K.; Oefner, P. J.; Vehreschild, J. J.; Vehreschild, M. J. G. T. |
| Article Title: | Assessment of urinary 3-indoxyl sulfate as a marker for gut microbiota diversity and abundance of Clostridiales |
| Abstract: | Objectives: After allogeneic hematopoietic stem cell transplantation (allo-HCT), urinary levels of 3-indoxyl sulfate (3-IS) correlate with the relative abundance of bacteria from the class Clostridia (RAC), and antibiotic treatment is considered the major determinant of this outcome. A high RAC has been associated with favorable outcome after allo-HCT and protection from Clostridium difficile infection (CDI). We assessed correlations between alpha diversity, RAC and urinary 3-IS levels in a non-allo-HCT clinical cohort of antibiotic treated patients to further explore 3-IS as a biomarker of reduced diversity and predisposition to CDI. Methods: Fecal and urinary specimens were analyzed from 40 non-allo-HCT hospitalized patients before and 9 ± 2 days after initiation of intravenous antibiotic treatment. Fecal microbiota were analyzed by 16s RNA sequencing and urinary 3-IS was analyzed by liquid chromatography-tandem mass spectrometry. Receiver operating characteristic (ROC) analysis was performed to assess the predictive value of 3-IS. Results: At a RAC cutoff of <30%, the binary logarithm of 3-IS (medium 3-IS: ≤2.5; high 3-IS: >2.5) was predictive with an accuracy of 82% (negative predictive value: 87%, positive predictive value 67%). Accuracy was improved by combing antibiotic history with 3-IS levels (accuracy 89%, npv 88%, ppv 92%). Conclusion: In conjunction with patient antibiotic history, 3-IS is a candidate marker to predict RAC. © 2018, © 2018 Taylor & Francis. |
| Keywords: | adult; clinical article; controlled study; middle aged; antibiotic therapy; sequence analysis; nonhuman; outcome assessment; biological marker; biomarker; allogeneic hematopoietic stem cell transplantation; clostridium difficile infection; urinalysis; intestine flora; disease predisposition; rna 16s; cotrimoxazole; macrolide; penicillin derivative; quinoline derived antiinfective agent; dna extraction; predictive value; feces analysis; cephalosporin; rna sequence; drug urine level; phylogeny; carbapenem; liquid chromatography-mass spectrometry; microbiota; microbial diversity; taxonomy; clostridiales; measurement accuracy; human; article; 3-indoxyl sulfate; alpha diversity; indican |
| Journal Title: | Gut Microbes |
| Volume: | 10 |
| Issue: | 2 |
| ISSN: | 1949-0976 |
| Publisher: | Taylor & Francis Group |
| Date Published: | 2019-01-01 |
| Start Page: | 133 |
| End Page: | 141 |
| Language: | English |
| DOI: | 10.1080/19490976.2018.1502536 |
| PUBMED: | 30118620 |
| PROVIDER: | scopus |
| PMCID: | PMC6546351 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 May 2019 -- Source: Scopus |
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