Genome-wide analysis of cancer/testis gene expression Journal Article
| Authors: | Hofmann, O.; Caballero, O. L.; Stevenson, B. J.; Chen, Y. T.; Cohen, T.; Chua, R.; Maher, C. A.; Panji, S.; Schaefer, U.; Kruger, A.; Lehvaslaiho, M.; Carninci, P.; Hayashizaki, Y.; Jongeneel, C. V.; Simpson, A. J. G.; Old, L. J.; Hide, W. |
| Article Title: | Genome-wide analysis of cancer/testis gene expression |
| Abstract: | Cancer/Testis (CT) genes, normally expressed in germ line cells but also activated in a wide range of cancer types, often encode antigens that are immunogenic in cancer patients, and present potential for use as biomarkers and targets for immunotherapy. Using multiple in silico gene expression analysis technologies, including twice the number of expressed sequence tags used in previous studies, we have performed a comprehensive genome-wide survey of expression for a set of 153 previously described CT genes in normal and cancer expression libraries. We find that although they are generally highly expressed in testis, these genes exhibit heterogeneous gene expression profiles, allowing their classification into testis-restricted (39), testis/brain-restricted (14), and a testis-selective (85) group of genes that show additional expression in somatic tissues. The chromosomal distribution of these genes confirmed the previously observed dominance of X chromosome location, with CT-X genes being significantly more testis-restricted than non-X CT. Applying this core classification in a genome-wide survey we identified >30 CT candidate genes; 3 of them, PEPP-2, OTOA, and AKAP4, were confirmed as testis-restricted or testis-selective using RT-PCR, with variable expression frequencies observed in a panel of cancer cell lines. Our classification provides an objective ranking for potential CT genes, which is useful in guiding further identification and characterization of these potentially important diagnostic and therapeutic targets. © 2008 by The National Academy of Sciences of the USA. |
| Keywords: | controlled study; human tissue; human cell; cancer patient; biological marker; reverse transcription polymerase chain reaction; gene expression profiling; computational biology; membrane proteins; homeodomain proteins; cancer cell culture; gene library; cell line, tumor; prediction; genome analysis; germ line; antigen; immunotherapy; gene identification; testicular neoplasms; genomics; x chromosome; chromosomes, human, x; tumor gene; chromosomes, human; testis; genome, human; electric potential; a kinase anchor proteins; gene index |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 105 |
| Issue: | 51 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 2008-12-23 |
| Start Page: | 20422 |
| End Page: | 20427 |
| Language: | English |
| DOI: | 10.1073/pnas.0810777105 |
| PUBMED: | 19088187 |
| PROVIDER: | scopus |
| PMCID: | PMC2603434 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 31" - "Export Date: 17 November 2011" - "CODEN: PNASA" - "Source: Scopus" |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work