Effectiveness of the combination of vascular targeted photodynamic therapy and anti-cytotoxic T-lymphocyte-associated antigen 4 in a preclinical mouse model of urothelial carcinoma Journal Article
| Authors: | Corradi, R. B.; LaRosa, S.; Jebiwott, S.; Murray, K. S.; Rosenzweig, B.; Somma, A. J.; Gomez, R. S.; Scherz, A.; Kim, K.; Coleman, J. A. |
| Article Title: | Effectiveness of the combination of vascular targeted photodynamic therapy and anti-cytotoxic T-lymphocyte-associated antigen 4 in a preclinical mouse model of urothelial carcinoma |
| Abstract: | Objective: To investigate the effectiveness of combination treatment of vascular targeted photodynamic therapy and anti-cytotoxic T-lymphocyte-associated antigen 4 immunotherapy in a mouse model of urothelial carcinoma. Methods: We used C57BL/6 mice injected with murine bladder 49 cell line. Mice were randomly allocated into four treatment groups: vascular targeted photodynamic therapy only, anti-cytotoxic T-lymphocyte-associated antigen 4 only, combination therapy and control. We carried out three separate experiments that used distinct cohorts of mice: tumor growth and development of lung metastases monitored with bioluminescent imaging (n = 91); survival evaluated with Kaplan–Meier curves (n = 111); and tumor cell population studied with flow cytometry (n = 20). In a fourth experiment, we re-challenged tumors in previously treated mice and compared tumor growth with that of naïve mice. Results: Combination therapy provided significant benefits over the other three treatment groups: prolonged survival (P < 0.0001), lower tumor signal (P < 0.0001) and decreased lung signal uptake (P ≤ 0.002). We also observed that mice previously treated with vascular targeted photodynamic therapy only or combination therapy did not present tumor growth after re-challenged tumors. Conclusions: Combination of vascular targeted photodynamic therapy with anti-cytotoxic T-lymphocyte-associated antigen 4 is an effective therapy in a urothelial carcinoma syngeneic mouse model. The present results suggest this therapy as a potential treatment option for both bladder and upper tract tumors in future clinical trials. © 2019 The Japanese Urological Association |
| Keywords: | urinary bladder neoplasms; photochemotherapy; cytotoxic t-lymphocyte-associated antigen 4; wst11 compound; mb-49 cell line |
| Journal Title: | International Journal of Urology |
| Volume: | 26 |
| Issue: | 3 |
| ISSN: | 0919-8172 |
| Publisher: | Wiley Blackwell |
| Date Published: | 2019-03-01 |
| Start Page: | 414 |
| End Page: | 422 |
| Language: | English |
| DOI: | 10.1111/iju.13878 |
| PUBMED: | 30659668 |
| PROVIDER: | scopus |
| PMCID: | PMC7583638 |
| DOI/URL: | |
| Notes: | Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
MSK Authors
Related MSK Work