Alteration in proliferative and endocrine responsiveness of human mammary carcinoma cells by prototypic tumor-suppressing agents Journal Article
| Authors: | Suto, A.; Bradlow, H. L.; Kubota, T.; Kitajima, M.; Wong, G. Y.; Osborne, M. P.; Telang, N. T. |
| Article Title: | Alteration in proliferative and endocrine responsiveness of human mammary carcinoma cells by prototypic tumor-suppressing agents |
| Abstract: | The experiments performed in this study were designed to establish that (1) acquisition of anchorage-independent growth, a biological characteristic of tumorigenically transformed phenotype, can be modulated by prototypic tumor-suppressing agents, and (2) modulation of growth is influenced by the metabolic competence of the cells to biotransform estradiol. MCF-7 human breast carcinoma cells exhibited linear cell proliferative kinetics with a 41-hour population doubling time, and a 15% colony-forming efficiency in 0.33% agar. Indole-3-carbinol (I3C), a naturally occurring tumor-suppressive agent; tamoxifen (TAM), an antiestrogenic agent; and 4-hydroxytamoxifen (4-OHTAM), a metabolite of TAM, demonstrated 73.7%, 72.5%, and 89.9% suppression in anchorage-independent growth of MCF-7 cells, respectively. At the metabolic level, 13C and 4-OHTAM induced 2.3-fold (P < 0.001) and 1.3-fold increase (P = 0.001) relative to their own controls in the extent of 2-hydroxylation of estradiol. The results indicate that growth inhibition by I3C, TAM, and 4-OMTAM may in part be due to altered estradiol metabolism in MCF-7 cells. Thus, anchorage-independent growth and altered biotransformation of estradiol may constitute useful cellular and endocrine markers to evaluate the biological response of chemosuppressive agents. © 1993. |
| Keywords: | controlled study; antineoplastic agents; cell proliferation; animal cell; cell division; tumor cells, cultured; breast neoplasms; animalia; cancer inhibition; isotope labeling; breast carcinoma; tamoxifen; cell marker; indoles; estradiol; cell strain mcf 7; steroid receptor; anchorage independent growth; carcinoma cell; 4 hydroxytamoxifen; growth inhibition; tritium; hydroxylation; steroids; cell kinetics; estrogen metabolism; human; article; mink cell focus-forming virus; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; 3 indolemethanol; steroid hydroxylation; chemosuppression; cloning-efficiency; estradiol metabolism; human mammary tumor cells |
| Journal Title: | Steroids |
| Volume: | 58 |
| Issue: | 5 |
| ISSN: | 0039-128X |
| Publisher: | Elsevier Inc. |
| Date Published: | 1993-05-01 |
| Start Page: | 215 |
| End Page: | 219 |
| Language: | English |
| DOI: | 10.1016/0039-128x(93)90021-e |
| PUBMED: | 8356573 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 March 2019 -- Source: Scopus |
