Identification of nine tissue-specific transcription factors of the hepatocyte nuclear factor 3/forkhead DNA-binding-domain family Journal Article
| Authors: | Clevidence, D. E.; Overdier, D. G.; Tao, W.; Qian, X.; Pani, L.; Lai, E.; Costa, R. H. |
| Article Title: | Identification of nine tissue-specific transcription factors of the hepatocyte nuclear factor 3/forkhead DNA-binding-domain family |
| Abstract: | Hepatocyte nuclear factor (HNF)-3α, -3β, and -3γ are liver transcription factors that mediate the coordinate expression of a number of hepatocyte-specific genes. The HNF-3 proteins share DNA-binding-domain homology among themselves and with the Drosophila homeotic protein forkhead (fkh). The HNF-3/fkh DNA-binding domain constitutes an uncharacterized protein motif that recognizes its cognate DNA binding site as a monomer. Additional HNF-3/fkh-related proteins are known to be required for determination events during embryogenesis in Drosophila and Xenopus. In this report, we describe the isolation of nine additional HNF-3/fkh homologue (HFH) clones from rodent tissue cDNAs by using both low-stringency hybridization and a polymerase chain reaction protocol. Many of the HFH genes exhibit a tissue-restricted expression pattern and are transcribed in tissues other than liver, including brain, kidney, lung, and intestine. The HNF-3/fkh motif therefore comprises a large gene family of transcription factors that play a role in tissue-specific gene regulation and development. |
| Keywords: | dna binding protein; dna-binding proteins; polymerase chain reaction; protein domain; animals; transcription factor; drosophila; gene library; transcription factors; nuclear proteins; rna; dna; amino acid sequence; molecular sequence data; sequence homology, amino acid; lung; organ specificity; base sequence; mutagenesis, site-directed; rats; rodentia; liver cell; blotting, northern; differentiation; multigene family; oligonucleotides, antisense; oligodeoxyribonucleotides; hepatocyte nuclear factor 3-beta; homeobox; gene family; restriction mapping; nuclear factor; dna hybridization; hepatocyte nuclear factor 3-alpha; humans; priority journal; article; tissue-specific transcription factors; hepatocyte nuclear factor 3-gamma |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 90 |
| Issue: | 9 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1993-05-01 |
| Start Page: | 3948 |
| End Page: | 3952 |
| Language: | English |
| DOI: | 10.1073/pnas.90.9.3948 |
| PUBMED: | 7683413 |
| PROVIDER: | scopus |
| PMCID: | PMC46423 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 March 2019 -- Source: Scopus |
