Nova, the paraneoplastic Ri antigen, is homologous to an RNA-binding protein and is specifically expressed in the developing motor system Journal Article
| Authors: | Buckanovich, R. J.; Posner, J. B.; Darnell, R. B. |
| Article Title: | Nova, the paraneoplastic Ri antigen, is homologous to an RNA-binding protein and is specifically expressed in the developing motor system |
| Abstract: | Paraneoplastic opsoclonus-ataxia, a disorder of motor control, develops in breast or lung cancer patients who harbor an antibody (Ri) that recognizes their tumors and a nuclear neuronal protein of 55 kd. We have characterized a gene, Nova, encoding an antigen recognized by the Ri antibody. Nova encodes a novel, highly conserved protein, homologous to the RNA-binding protein hnRNP K, the yeast splicing protein MER1, and a motif in several retroviral proteases. Northern blot analysis detects Nova transcripts only in brain, and several alternatively spliced forms are present in brain and tumor cells. Nova expression is restricted to the ventral brain stem and spinal cord in E18 mice. Since Nova encodes a target antigen in the motor disorder paraneoplastic opsoclonus-ataxia that is expressed in the developing subcortical motor system, it is a likely participant in both the pathogenesis of paraneoplastic opsoclonus-ataxia and the developmental biology of the motor system. The homology between Nova and hnRNP K suggests that Nova regulates RNA splicing or metabolism in a specific subset of developing neurons. © 1993. |
| Keywords: | immunohistochemistry; human tissue; human cell; comparative study; polymerase chain reaction; antigen expression; protein analysis; cerebellum; breast cancer; gene expression; lung neoplasms; lung cancer; tumor cells, cultured; rna binding protein; gene expression regulation; rna-binding proteins; liver; amino acid sequence; conserved sequence; molecular sequence data; sequence homology, amino acid; antigen; brain; ribonucleoproteins; base sequence; development; dna primers; sequence homology; motor dysfunction; paraneoplastic syndrome; blotting, northern; brain stem; gene location; gene structure; northern blotting; carcinoma, small cell; hippocampus; motor system; nerve cell necrosis; consensus sequence; open reading frames; paraneoplastic syndromes; human; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s. |
| Journal Title: | Neuron |
| Volume: | 11 |
| Issue: | 4 |
| ISSN: | 0896-6273 |
| Publisher: | Cell Press |
| Date Published: | 1993-10-01 |
| Start Page: | 657 |
| End Page: | 672 |
| Language: | English |
| DOI: | 10.1016/0896-6273(93)90077-5 |
| PUBMED: | 8398153 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 March 2019 -- Source: Scopus |
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