Nuclear magnetic resonance structural studies of A · AT base triple alignments in intramolecular purine · purine · pyrimidine DNA triplexes in solution Journal Article


Authors: Radhakrishnan, I.; de los Santos, C.; Patel, D. J.
Article Title: Nuclear magnetic resonance structural studies of A · AT base triple alignments in intramolecular purine · purine · pyrimidine DNA triplexes in solution
Abstract: This study reports on an NMR characterization of an intramolecular purine · purine · pyrimidine (R · RY) triplex containing a, central A · AT triple in addition to G · GC and T · AT triples. Our studies establish an A(anti) · AT alignment with reversed Hoogsteen pairing stabilized by two N6H . . . N hydrogen bonds between adenine residues in the third strand and the purine strand of the duplex. This result, combined with our earlier demonstration of G(anti) · GC and T(anti) · AT pairing alignments, has provided a definitive experimental approach for differentiating between the base triple pairing possibilities proposed previously by Beal and Dervan for R · RY triplexes. © 1993 Academic Press Limited.
Keywords: pyrimidines; dna; magnetic resonance spectroscopy; dna structure; hydrogen bonding; purines; nucleic acid conformation; nuclear magnetic resonance; nmr; nucleic acid base; priority journal; article; support, u.s. gov't, p.h.s.; deoxyribonucleotides; a · at triples; r · ry triplexes; triple helix
Journal Title: Journal of Molecular Biology
Volume: 234
Issue: 1
ISSN: 0022-2836
Publisher: Academic Press Inc., Elsevier Science  
Date Published: 1993-11-05
Start Page: 188
End Page: 197
Language: English
DOI: 10.1006/jmbi.1993.1573
PUBMED: 8230198
PROVIDER: scopus
DOI/URL:
Notes: Article -- Export Date: 1 March 2019 -- Source: Scopus
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  1. Dinshaw J Patel
    478 Patel