Solution structure of a pyrimidine·purine·pyrimidine DNA triplex containing T·AT, C(+) ·GC and G·TA triples Journal Article

Authors: Radhakrishnan, I.; Patel, D. J.
Article Title: Solution structure of a pyrimidine·purine·pyrimidine DNA triplex containing T·AT, C(+) ·GC and G·TA triples
Abstract: Background Under certain conditions, homopyrimidine oligonucleotides can bind to complementary homopurine sequences in homopurine-homopyrimidine segments of duplex DNA to form triple helical structures. Besides having biological implications in vivo, this property has been exploited in molecular biology applications. This approach is limited by a lack of knowledge about the recognition by the third strand of pyrimidine residues in Watson-Crick base pairs. Results We have therefore determined the solution structure of a pyrimidine·purine·pyrimidine (Y·RY) DNA triple helix containing a guanine residue in the third strand which was postulated to specifically recognize a thymine residue in a Watson-Crick TA base pair. The structure was solved by combining NMR-derived restraints with molecular dynamics simulations conducted in the presence of explicit solvent and counter ions. The guanine of the G·TA triple is tilted out of the plane of its target TA base pair towards the 3′-direction, to avoid a steric clash with the thymine methyl group. This allows the guanine amino protons to participate in hydrogen bonds with separate carbonyls, forming one strong bond within the G·TA triple and a weak bond to an adjacent T·AT triple. Dramatic variations in helical twist around the guanine residue lead to a novel stacking interaction. At the global level, the Y·RY DNA triplex shares several structural features with the recently solved solution structure of the R·RY DNA triplex. Conclusions The formation of a G·TA triple within an otherwise pyrimidine·purine·pyrimidine DNA triplex causes conformational realignments in and around the G·TA triple. These highlight new aspects of molecular recognition that could be useful in triplex-based approaches to inhibition of gene expression and site-specific cleavage of genomic DNA. © 1994 Elsevier Science Ltd. All rights reserved.
Keywords: molecular genetics; methodology; molecular dynamics; pyrimidines; chemistry; dna; molecular sequence data; nucleotide sequence; magnetic resonance spectroscopy; nuclear magnetic resonance spectroscopy; base sequence; models, molecular; solutions; purines; chemical structure; purine derivative; conformation; nucleic acid conformation; pyrimidine derivative; synthesis; solution and solubility; macromolecule; oligodeoxyribonucleotides; stress, mechanical; base composition; mechanical stress; nmr spectroscopy; article; oligodeoxyribonucleotide; support, u.s. gov't, p.h.s.; macromolecular systems; dna base composition; g·ta triple; pyrimidine·purine·pyrimidine dna triplex
Journal Title: Structure
Volume: 2
Issue: 1
ISSN: 0969-2126
Publisher: Cell Press  
Date Published: 1994-01-01
Start Page: 17
End Page: 32
Language: English
DOI: 10.1016/s0969-2126(00)00005-8
PROVIDER: scopus
PUBMED: 8075980
Notes: Structure -- Cited By :108 -- Export Date: 14 January 2019 -- Article -- CODEN: STRUE C2 - 8075980 -- Source: Scopus
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MSK Authors
  1. Dinshaw J Patel
    445 Patel