Development of a method to measure kinetics of radiolabelled monoclonal antibody in human tumour with applications to microdosimetry: Positron emission tomography studies of iodine-124 labelled 3F8 monoclonal antibody in glioma Journal Article

  

Authors:	Daghighian, F.; Pentlow, K. S.; Larson, S. M.; Graham, M. C.; DiResta, G. R.; Yeh, S. D. J.; Macapinlac, H.; Finn, R. D.; Arbit, E.; Cheung, N. K. V.
Article Title:	Development of a method to measure kinetics of radiolabelled monoclonal antibody in human tumour with applications to microdosimetry: Positron emission tomography studies of iodine-124 labelled 3F8 monoclonal antibody in glioma
Abstract:	We present a method to assess quantitatively the immunological characteristics of tumours using radiolabelled monoclonal antibody and positron emission tomography (PET) to improve dosimetry for radioimunotherapy. This method is illustrated with a glioma patient who was injected with 96.2 MBq of iodine-124 labelled 3F8, a murine antibody (IgG3) specific against the ganglioside GD2. Serial PET scans and plasma samples were taken over 11 days. A three-compartment model was used to estimate the plasma to tumour transfer constant (K1), the tumour to plasma transfer constant k2, the association and dissociation constants (k3, k4) of antibody binding, and the binding potential. Tumour radioactivity peaked at 18 h at 0.0045% ID/g. The kinetic parameters were estimated to be: K1 = 0.048 ml h-1 g-1, k2 = 0.16 h-1, k3 = 0.03 h-1, k4 = 0.015 h-1 and BP = 2.25. Based on these kinetic parameters, the amount of tumour-bound radiolabelled monoclonal antibody was calculated. This method permits estimates of both macrodosimetry and microdosimetry at the cellular level based on in vivo non-invasive measurement. © 1993 Springer-Verlag.
Keywords:	case report; positron emission tomography; glioma; brain neoplasms; radiotherapy dosage; models, theoretical; monoclonal antibody; iodine radioisotopes; brain; radiopharmaceutical agent; iodine 124; drug blood level; antigen binding; radioimmunotherapy; drug transport; antibody; drug binding; intravenous drug administration; tomography, emission-computed; compartment model; dissociation constant; tumor immunology; radioimmunodetection; microdosimetry; iodine-124; human; article; support, non-u.s. gov't; support, u.s. gov't, non-p.h.s.
Journal Title:	European Journal of Nuclear Medicine
Volume:	20
Issue:	5
ISSN:	0340-6997
Publisher:	Springer Verlag
Date Published:	1993-05-01
Start Page:	402
End Page:	409
Language:	English
DOI:	10.1007/bf00208998
PUBMED:	8519259
PROVIDER:	scopus
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027302774&doi=10.1007%2fBF00208998&partnerID=40&md5=1bd9bd345e89b35b338487034eed1d6b
Notes:	Source: Scopus

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