| Abstract: |
The T cell response to allogeneic murine Mls determinants is not H‐2 restricted but is dependent on H‐2 class II molecules on the Mls‐expressing stimulator cells. We have tested planar membranes containing H‐2 class II I‐E molecules alone or with I‐A molecules for their ability to activate a panel of Mls1a‐specific T hybrids. Despite the ability of the planar membranes to activate an alloreactive T hybrid and to present staphylococcal enterotoxins or an antigenic peptide to appropriately responsive T hybrids, they failed to stimulate the Mls1a‐specific T hybrids. These findings, in the light of the various controls demonstrating sufficiency of the I‐E molecules in the planar membranes, indicate that Mls1a determinants are not covalently bound to I‐E molecules; the two molecular species are thus either not physically associated or are linked by a relatively weak interaction. In addition, our experiments show that isolated I‐E molecules but not I‐A molecules present staphylococcal enterotoxins A and B to two independently derived T hybrids expressing T cell receptor Vβ1, Vβ8.2 and Vβ6 elements. Copyright © 1993 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim |
| Keywords: |
controlled study; nonhuman; t-lymphocytes; animal cell; mouse; animal; mice; peptide; mice, inbred strains; t lymphocyte receptor beta chain; antigen presentation; lymphocyte activation; molecular interaction; staphylococcus aureus; t lymphocyte activation; membrane binding; membrane; antigens, bacterial; hybrid cell; superantigen; enterotoxins; staphylococcus enterotoxin b; h-2 antigens; planar membranes; priority journal; article; h2 antigen; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; staphylococcus enterotoxin a; mls; purified class ii; t hybrids; staphylococcus enterotoxin; minor lymphocyte stimulatory antigens
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