Interphase cytogenetics of gastric and esophageal adenocarcinomas Journal Article
| Authors: | Rao, P. H.; Mathew, S.; Lauwers, G.; Rodriguez, E.; Kelsen, D. P.; Chaganti, R. S. K. |
| Article Title: | Interphase cytogenetics of gastric and esophageal adenocarcinomas |
| Abstract: | Numerical changes affecting chromosomes 1,2,3,4,6,7, 8, 10, 11, 12, 16, 17, 18, and the X and Y chromosomes have been analyzed using chromosome-specific centro-meric a-satellite repeat DNA probes in a panel of biopsies of six gastric and three esophageal adenocarcinoma and one epidermoid carcinoma of esophagus obtained at surgery. For each case, with each probe, the number of hybridization signals were determined in 200 nuclei. Hybridization of each probe to phytohemagglutinin-stimulated normal peripheral blood lymphocytes served as controls. Monosomy was defined by loss of one signal in 15% or more cells and trisomy or tetrasomy was defined by the presence of 3 or 4 signals in 7% or more cells, respectively. The Y chromosome was lost in 6 of 8 cases and monosomy 10 was seen in 5 of 10 cases. Trisomy for chromosomes 17, 8, 7, 12, 11, and 1 was seen in 4 of 10, 4 of 10, 4 of 10, 2 of 10, 2 of 8, and 2 of 10 cases, respectively, and tetrasomy for chromosome 7 was seen in 1 of 10 cases. These data show that the Y chromosome and chromosomes 10, 8, 7, 17, and 12 are most frequently involved in nondisjunctional changes in these tumors. They also document the feasibility and utility of interphase cytogenetics of gastric adenocarcinomas. © 1993 Raven-press. Ltd. |
| Keywords: | adult; clinical article; controlled study; human tissue; aged; aged, 80 and over; adenocarcinoma; in situ hybridization, fluorescence; cytogenetics; chromosomes, human, pair 8; dna, neoplasm; chromosome aberrations; stomach adenocarcinoma; chromosome loss; stomach neoplasms; esophagus carcinoma; esophagus; esophageal neoplasms; chromosomes; stomach; nondisjunction; y chromosome; chromosomes, human, pair 17; middle age; trisomy; chromosome 10; interphase; monosomy; chromosomes, human, pair 7; ploidies; dna probes; chromosome disorders; human; male; female; priority journal; article; support, u.s. gov't, p.h.s.; dna probe; tetrasomy |
| Journal Title: | Diagnostic Molecular Pathology |
| Volume: | 2 |
| Issue: | 4 |
| ISSN: | 1052-9551 |
| Publisher: | Lippincott Williams & Wilkins |
| Date Published: | 1993-12-01 |
| Start Page: | 264 |
| End Page: | 268 |
| Language: | English |
| DOI: | 10.1097/00019606-199312000-00006 |
| PUBMED: | 8118604 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Source: Scopus |
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