Transmeiotic differentiation of male germ cells in culture Journal Article

  


Authors: Rassoulzadegan, M.; Paquis-Flucklinger, V.; Bertino, B.; Sage, J.; Jasin, M.; Miyagawa, K.; van Heyningen, V.; Besmer, P.; Cuzin, F.
Article Title: Transmeiotic differentiation of male germ cells in culture
Abstract: A cell culture system that supports the differentiation of male germ cells through meiosis is described. It takes advantage of the properties of a cell line, 15P-1,. established from testicular cells of transgenic mice that express the large T protein of polyoma virus in the seminiferous epithelium. This line exhibits features characteristic of Sertoli cells, including transcription of the Wilms' tumor (WT1) and Steel genes. Cells of the 15P-1 type support the meiotic and postmeiotic differentiation in cocultures of diploid premeiotic germ cells into haploid spermatids expressing the protamine (Prm-1) gene. When cocultured with 15P-1 cells, testicular cells explanted from immature 9-day-old animals, before the onset of the first meiosis, generated tetrads of haploid cells with the morphology of round spermatids and initiated protamine transcription. © 1993.
Keywords: controlled study; dna-binding proteins; nonhuman; animal cell; mouse; animal; meiosis; mice; cells, cultured; stem cell factor; gene expression; germ cell; genetic transcription; cell differentiation; transgenic mouse; animalia; mus musculus; mice, transgenic; molecular sequence data; cell culture; rna, messenger; virus large t antigen; base sequence; polyoma virus; polyomavirus; dna primers; antigens, polyomavirus transforming; in vitro; nephroblastoma; spermatogenesis; wt1 proteins; sertoli cell; sertoli cells; protamine; testis cell; haploidy; spermatid; male; priority journal; article; support, non-u.s. gov't; hematopoietic cell growth factors; protamines
Journal Title: Cell
Volume: 75
Issue: 5
ISSN: 0092-8674
Publisher: Cell Press  
Date Published: 1993-12-03
Start Page: 997
End Page: 1006
Language: English
DOI: 10.1016/0092-8674(93)90543-y
PUBMED: 7504588
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027756761&doi=10.1016%2f0092-8674%2893%2990543-Y&partnerID=40&md5=bc1e4ccd83e7d9e9be6961b1a898cebc
Notes: Source: Scopus
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MSK Authors
  1. Maria Jasin
    249 Jasin
  2. Peter Besmer
    115 Besmer
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