Molecular cloning of a complementary DNA encoding a prostate-specific membrane antigen Journal Article
| Authors: | Israeli, R. S.; Powell, C. T.; Fair, W. R.; Heston, W. D. W. |
| Article Title: | Molecular cloning of a complementary DNA encoding a prostate-specific membrane antigen |
| Abstract: | Recently, a novel Mr 100,000 prostate-specific membrane glycoprotein (PSM) has been detected by the prostate-specific monoclonal antibody 7E11-C5, raised against the human prostatic carcinoma cell line LNCaP. The PSM antigen is expressed exclusively by normal and neoplastic prostate cells and metastases. We now report the molecular cloning of a full-length 2.65-kilobase complementary DNA encoding the PSM antigen from a human LNCaP complementary DNA library by polymerase chain reaction using degenerate oligonucleotide primers. Analysis of the complementary DNA sequence has revealed that a portion of the coding region, from nucleotide 1250 to 1700, has 54% homology to the human transferrin receptor mRNA. The deduced polypeptide has a putative transmembrane domain enabling the delineation of intra- and extracellular portions of this antigen. In contrast to prostate-specific antigen and prostatic acid phosphatase which are secreted proteins, PSM as an integral membrane protein may prove to be effective as a target for imaging and cytotoxic targeting modalities. © 1993, American Association for Cancer Research. All rights reserved. |
| Keywords: | human cell; polymerase chain reaction; protein domain; antigen expression; prostate specific antigen; cancer cell culture; monoclonal antibody; rna; molecular cloning; cloning, molecular; prostate; dna; amino acid sequence; molecular sequence data; membrane glycoproteins; antigen detection; membrane protein; peptides; immunoprecipitation; base sequence; dna sequence; sequence homology; complementary dna; prostate carcinoma; northern blotting; transferrin receptor; dna library; acid phosphatase prostate isoenzyme; precipitin tests; human; male; priority journal; article; support, u.s. gov't, p.h.s. |
| Journal Title: | Cancer Research |
| Volume: | 53 |
| Issue: | 2 |
| ISSN: | 0008-5472 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 1993-01-15 |
| Start Page: | 227 |
| End Page: | 230 |
| Language: | English |
| PUBMED: | 8417812 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Source: Scopus |
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