Morphological transformation of Syrian hamster embryo cells in primary culture by malachite green correlates well with the evidence for formation of reactive free radicals Journal Article
| Authors: | Panandiker, A.; Fernandes, C.; Gundu Rao, T. K.; Kesava Rao, K. V. |
| Article Title: | Morphological transformation of Syrian hamster embryo cells in primary culture by malachite green correlates well with the evidence for formation of reactive free radicals |
| Abstract: | Malachite green (MG) (green crystals with metallic luster and very soluble in water) is highly cytotoxic to mammalian cells and also acts as a liver tumor promoter. In view of its industrial importance and possible exposure to individuals, MG poses a potential environmental health hazard. We have studied the effect of MG on the formation of morphologically transformed colonies using Syrian hamster embryo (SHE) cell transformation assay. MG induced a dose-related increase in the formation of transformed foci, the optimum concentration being 0.05 μg/ml. Electron spin resonance (ESR) analysis using 5,5-dimethyl-1-pyrroline N-oxide (DMPO) as a spin-trapping agent showed the formation of reactive free radicals during the in vitro metabolism of MG. The present study suggests a close relationship between the morphological transformation of SHE cells by MG and the possible involvement of reactive free radical formation. © 1993. |
| Keywords: | controlled study; dose response; nonhuman; rosaniline dyes; animal cell; animal; metabolism; mammalia; cells, cultured; cell structure; embryo; dose-response relationship, drug; malachite green; animalia; cell transformation, neoplastic; cell culture; cell transformation; cell line, transformed; spin labels; embryo cell; biotransformation; mutagens; carcinogens; cricetinae; occupational exposure; free radicals; electron spin resonance; electron spin resonance spectroscopy; mutagenicity tests; free radical; cyclic n-oxides; priority journal; article; mesocricetus; hamsters; mesocricetus auratus; syrian hamster; electron spin resonance (esr) analysis; free radical formation; morphological transformation; spin-trapping agent; syrian hamster embryo cells; 5,5 dimethyl 1 pyrroline 1 oxide; industrial chemical; siproeta stelenes |
| Journal Title: | Cancer Letters |
| Volume: | 74 |
| Issue: | 1-2 |
| ISSN: | 0304-3835 |
| Publisher: | Elsevier Ireland Ltd. |
| Date Published: | 1993-10-15 |
| Start Page: | 31 |
| End Page: | 36 |
| Language: | English |
| DOI: | 10.1016/0304-3835(93)90040-g |
| PUBMED: | 8287369 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Source: Scopus |
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