NMR structural studies on a nonnatural deoxyribonucleoside which mediates recognition of GC base pairs in pyrimidine·purine·primidine DNA triplexes Journal Article


Authors: Radhakrishnan, I.; Patel, D. J.; Priestley, E. S.; Nash, H. M.; Dervan, P. B.
Article Title: NMR structural studies on a nonnatural deoxyribonucleoside which mediates recognition of GC base pairs in pyrimidine·purine·primidine DNA triplexes
Abstract: As a part of our ongoing efforts to define the structural aspects of unusual pairing alignments in DNA triplexes by nuclear magnetic resonance spectroscopy, we have examined the structural role of a nonnatural deoxyribonucleoside, PI, that has been shown to mediate the recognition of GC base pairs in pyrimidine·purine·pyrimidine DNA triplexes [Koh, J. S., & Dervan, P. B. (1992) J. Am. Chem Soc. 114, 1470]. A qualitative interpretation of the NMR data indicates that this analog of protonated cytosine is readily accommodated in the third strand segment of an intramolecular triplex system. Furthermore, the observed NOE patterns position the imino and amino protons of PI opposite the N7 and O6 atoms of guanine, respectively, consistent with the previously proposed pairing scheme. © 1993, American Chemical Society. All rights reserved.
Keywords: dna; molecular sequence data; guanine; magnetic resonance spectroscopy; base sequence; base pairing; chemical structure; molecular structure; molecular interaction; dna helix; nuclear magnetic resonance; hydrogen-ion concentration; cytosine; base composition; priority journal; article; support, u.s. gov't, p.h.s.; support, u.s. gov't, non-p.h.s.; deoxyribonucleoside; deoxyribonucleosides
Journal Title: Biochemistry
Volume: 32
Issue: 41
ISSN: 0006-2960
Publisher: American Chemical Society  
Date Published: 1993-10-01
Start Page: 11228
End Page: 11234
Language: English
DOI: 10.1021/bi00092a037
PUBMED: 8218188
PROVIDER: scopus
DOI/URL:
Notes: Source: Scopus
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  1. Dinshaw J Patel
    394 Patel