Synthesis of a tissue-engineered periosteum with acellular dermal matrix and cultured mesenchymal stem cells Journal Article
| Authors: | Schonmeyr, B.; Clavin, N.; Avraham, T.; Longo, V.; Mehrara, B. J. |
| Article Title: | Synthesis of a tissue-engineered periosteum with acellular dermal matrix and cultured mesenchymal stem cells |
| Abstract: | Periosteal grafts can aid in bone repair by providing bone progenitor cells and acting as a barrier to scar tissue. Unfortunately, these grafts have many of the same disadvantages as bone grafts (donor site morbidity and limited donor sites). In this article, we describe a method of synthesizing a periosteum-like material using acellular human dermis and osteoblasts or mesenchymal stem cells (MSC). We show that osteoblasts readily attach to and proliferate on the acellular human dermis in vitro. In addition, osteoblasts retained the potential for differentiation in response to bone morphogenetic protein stimulation. Cells grown on the acellular human dermis were efficiently transfected with adenoviruses with no evidence of cellular toxicity. To assess for in vivo cell delivery and bone-forming potential, the acellular human dermis was seeded with green fluorescent protein (GFP)-positive MSCs, transfected with bone morphogenetic protein 2, wrapped around the adductor muscle in syngeneic mice, and used to treat critical-sized mandibular defects in nude rats. After 3 weeks, GFP-positive cells were still present, and bone had replaced the interface between the muscle and the constructs. After 6 weeks, critical-sized bone defects had been successfully healed. In conclusion, we show that an acellular human dermis can be used to synthesize a tissue-engineered periosteum capable of delivering cells and osteoinductive proteins. © Copyright 2009, Mary Ann Liebert, Inc. |
| Keywords: | controlled study; bone graft; nonhuman; cell proliferation; proteins; animal cell; mouse; animals; mice; animal tissue; cells, cultured; mus; green fluorescent protein; animal experiment; cell differentiation; cytotoxicity; periosteum; mice, inbred c57bl; dermis; extracellular matrix; wound healing; genetic transfection; cell culture; rat; bone; cell membranes; collagen; mesenchymal stem cell; in-vivo; rats; ossification; osteogenesis; rattus; animals, newborn; neovascularization, physiologic; in-vitro; cell adhesion; tissue engineering; synthesis (chemical); osteoblast; osteoblasts; progenitor cell; mandible; bone defect; adenoviridae; muscle; bone morphogenetic protein-2; bone morphogenetic proteins; bone repair; cell delivery; cellular toxicities; dermal matrix; human dermis; mandibular defect; osteoinductive protein; scar tissues; bone cement; flowcharting; bone regeneration; gene transfer techniques; mesenchymal stem cells; rats, inbred f344 |
| Journal Title: | Tissue Engineering Part A |
| Volume: | 15 |
| Issue: | 7 |
| ISSN: | 1937-3341 |
| Publisher: | Mary Ann Liebert, Inc |
| Date Published: | 2009-07-01 |
| Start Page: | 1833 |
| End Page: | 1841 |
| Language: | English |
| DOI: | 10.1089/ten.tea.2008.0446 |
| PUBMED: | 19125645 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Export Date: 30 November 2010" - "Source: Scopus" |
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