Immunohistochemical detection of bone morphogenetic proteins in bone and soft‐tissue sarcomas Journal Article
| Authors: | Yoshikawa, H.; Rettig, W. J.; Lane, J. M.; Takaoka, K.; Alderman, E.; Rup, B.; Rosen, V.; Healey, J. H.; Huvos, A. G.; Garin‐Chesa, P. |
| Article Title: | Immunohistochemical detection of bone morphogenetic proteins in bone and soft‐tissue sarcomas |
| Abstract: | Background. Bone morphogenetic proteins (BMPs) are potent inducers of bone formation. Functional and immunohistochemical studies have identified BMPs in a subset of osteosarcomas. In the present study, the authors extend the analysis of BMP expression to other bone and soft tissue sarcomas. Methods. Monoclonal antibody AbH3b2/17 against human BMP‐2 and BMP‐4 was used in avidin‐biotin‐immunoperoxidase assays with frozen sections of bone tumors (71 specimens), soft tissue sarcomas (69 specimens), and normal tissues. Results. Among bone tumors, BMP was detected in osteosarcomas (17 of 29 samples), malignant fibrous histiocytomas (MFHs) (6 of 6), and the spindle cell sarcomatous components of spindle cell (dedifferentiated) chondrosarcomas (4 of 4), but not in conventional chondrosarcomas (0 of 10) or Ewing's sarcomas (0 of 14). Histologic subtypes of osteosarcoma differed for BMP expression, with 8 of 9 fibrohistiocytic, 9 of 13 osteoblastic, and 0 of 5 chondroblastic lesions showing immunostaining. In all BMP‐positive bone tumors, immunostaining was localized in the cytoplasm of primitive mesenchymal cells, with little or no staining in tumor matrix and more mature osteoblastic/chondrocytic cells. Among soft tissue sarcomas, MFHs (11 of 12), liposarcomas (5 of 11), leiomyosarcomas (3 of 9), and malignant schwannomas (3 of 8) showed cytoplasmic BMP immunostaining. Synovial sarcomas (0 of 9), rhabdomyosarcomas (0 of 8), and fibrosarcomas (0 of 7) were BMP‐negative. All normal humantissues tested, including the tissues of a 16‐week‐old fetus, lacked BMP immunoreactivity. Conclusions. Bone morphogenetic protein is expressed in a subset of osteosarcomas, a high proportion of MFHs of bone and soft tissue, and in spindle cell chondrosarcomas. In these tumors, BMP is localized predominantly to the cytoplasm of malignant cells with primitive mesenchymal features; no or little BMP is detected in the more differentiated elements of bone and soft tissue sarcomas, Different histologic types of bone and soft tissue sarcomas may mimic discrete stages of mesenchymal differentiation as defined by BMP expression and histologic criteria. Copyright © 1994 American Cancer Society |
| Keywords: | immunohistochemistry; osteosarcoma; clinical article; controlled study; human tissue; bone neoplasms; human cell; histopathology; protein localization; proteins; protein analysis; bone morphogenetic protein; neoplasm proteins; monoclonal antibody; sarcoma; antibodies, monoclonal; fibrosarcoma; soft tissue sarcoma; ossification; leiomyosarcoma; malignant fibrous histiocytoma; rhabdomyosarcoma; sarcoma, synovial; chondrosarcoma; mesenchyme; soft tissue neoplasms; liposarcoma; biotin; bone morphogenetic proteins; neurilemmoma; bone structure; avidin; immunoperoxidase staining; human; priority journal; article; spindle cell chondrosarcoma; histiocytoma, fibrous |
| Journal Title: | Cancer |
| Volume: | 74 |
| Issue: | 3 |
| ISSN: | 0008-543X |
| Publisher: | Wiley Blackwell |
| Date Published: | 1994-08-01 |
| Start Page: | 842 |
| End Page: | 847 |
| Language: | English |
| DOI: | 10.1002/1097-0142(19940801)74:3<842::Aid-cncr2820740309>3.0.Co;2-b |
| PROVIDER: | scopus |
| PUBMED: | 8039112 |
| DOI/URL: | |
| Notes: | Export Date: 14 January 2019 -- Article -- Source: Scopus |
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85Rettig -
66Lane
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