Expression of bone morphogenetic proteins in human osteosarcoma: Immunohistochemical detection with monoclonal antibody Journal Article

  


Authors: Yoshikawa, H.; Rettig, W. J.; Takaoka, K.; Alderman, E.; Rup, B.; Rosen, V.; Wozney, J. M.; Lane, J. M.; Huvos, A. G.; Garin‐Chesa, P.
Article Title: Expression of bone morphogenetic proteins in human osteosarcoma: Immunohistochemical detection with monoclonal antibody
Abstract: Background. Bone morphogenetic proteins (BMP) induce ectopic bone formation in vivo and may play a role in normal bone development. In addition, bone morphogenetic activity, as measured in a bone‐forming assay in immunodeficient, athymic nu/nu mice, is present in a proportion of osteosarcomas; this activity, which may be mediated by BMP, is correlated with a poor prognosis. Methods. The development of a monoclonal antibody against recombinant human BMP‐2, AbH3b2/17, has allowed immunohistochemical localization of BMP in tumor tissues. Cryostat sections of osteosarcomas (21 tumor samples), chondrosarcomas (5 samples), and Ewing's sarcomas of bone (5 samples) were examined with AbH3b2/17 using the avidin‐biotin‐immunoperoxidase method. Results. The authors found AbH3b2/17 immunoreactivity in 12 of the 21 osteosarcoma samples (57% sensitivity) obtained from 20 patients. For one patient, samples of the primary lesion and a subsequent metastasis were tested, and only the latter showed AbH3b2/17 immunore‐activity. The chondrosarcomas and Ewing's sarcomas examined showed no immunoreactivity. In antigen‐positive osteosarcomas, AbH3b2/17 immunostaining was localized predominantly in the cytoplasm of tumor cells. Moreover, the proportion of AbH3b2/17‐reactive cells varied among osteosarcomas with disparate histologic features. Conclusions. The authors identified a rapid and widely applicable method for detecting BMP expression in intact tissues, which may complement and enhance the bone‐forming assay in nu/nu mice as a prognostic procedure in osteosarcomas. Copyright © 1994 American Cancer Society
Keywords: immunohistochemistry; osteosarcoma; adolescent; adult; child; clinical article; human tissue; aged; aged, 80 and over; bone neoplasms; proteins; bone morphogenetic protein; neoplasm proteins; monoclonal antibody; ewing sarcoma; gene expression regulation, neoplastic; antibodies, monoclonal; species specificity; protein synthesis; ossification; sarcoma, ewing's; chondrosarcoma; protein determination; staining and labeling; bone morphogenetic proteins; growth substances; middle age; prognosis; human; male; female; priority journal; article
Journal Title: Cancer
Volume: 73
Issue: 1
ISSN: 0008-543X
Publisher: Wiley Blackwell  
Date Published: 1994-01-01
Start Page: 85
End Page: 91
Language: English
DOI: 10.1002/1097-0142(19940101)73:1<85::Aid-cncr2820730116>3.0.Co;2-8
PROVIDER: scopus
PUBMED: 7506120
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028044723&doi=10.1002%2f1097-0142%2819940101%2973%3a1%3c85%3a%3aAID-CNCR2820730116%3e3.0.CO%3b2-8&partnerID=40&md5=1d9cc464f0acaf8181eee6e4d3803800
Notes: Export Date: 14 January 2019 -- Article -- Source: Scopus
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MSK Authors
  1. Andrew G Huvos
    289 Huvos
  2. Pilar Garin-Chesa
    73 Garin-Chesa
  3. Wolfgang J. Rettig
    85 Rettig
  4. Joseph M. Lane
    66 Lane
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