Cellular program controlling the recovery of adipose tissue mass: An in vivo imaging approach Journal Article
| Authors: | Birsoy, K.; Soukas, A.; Torrens, J.; Ceccarini, G.; Montez, J.; Maffei, M.; Cohen, P.; Fayzikhodjaeva, G.; Viale, A.; Socci, N. D.; Friedman, J. M. |
| Article Title: | Cellular program controlling the recovery of adipose tissue mass: An in vivo imaging approach |
| Abstract: | The cellular program responsible for the restoration of adipose tissue mass after weight loss is largely uncharacterized. Leptin mRNA levels are highly correlated with adipose tissue mass, and leptin expression can thus be used as a surrogate for changes in the amount of adipose tissue. To further study the responses of adipocytes to changes in weight, we created a transgenic mouse expressing the luciferase reporter gene under the control of leptin regulatory sequences, which allows noninvasive imaging of the leptin expression of mice in vivo. We used these animals to show that weight loss induced by fasting or leptin treatment results in the retention of lipid-depleted adipocytes in adipose depots. To further study the cellular response to weight regain after leptin treatment, a leptin withdrawal protocol was used to induce a state of acute leptin deficiency in wild type mice. Acute leptin deficiency led to the transient deposition of large amounts of glycogen within pre-existing, lipid-depleted adipocytes. This was followed by rapid reaccumulation of lipid. Transcriptional profiling revealed that this cellular response was associated with induction of mRNAs for the entire pathway of enzymes necessary to convert glucose into acetyl-CoA and glycerol, key substrates for the synthesis of triglycerides. © 2008 by The National Academy of Sciences of the USA. |
| Keywords: | gene sequence; nonhuman; leptin; mouse; animals; mice; animal tissue; mus; cell function; gene expression; luciferase; animal experiment; body weight; feeding behavior; genetic transcription; in vivo study; transcription, genetic; enzyme substrate; wild type; mice, inbred c57bl; transgenic mouse; animalia; mus musculus; whole body imaging; organ specificity; reporter gene; gene control; triacylglycerol; lipids; protein deficiency; cell cycle regulation; luciferases; organ size; non invasive measurement; lipogenesis; diet restriction; fasting; adipose tissue; adipocyte; glycerol; animals, genetically modified; weight change; adipocytes; acetyl coenzyme a; cellular stress signal; glycogen metabolism; leptin deficiency; lipid storage; glycogen |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 105 |
| Issue: | 35 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 2008-09-02 |
| Start Page: | 12985 |
| End Page: | 12990 |
| Language: | English |
| DOI: | 10.1073/pnas.0805621105 |
| PUBMED: | 18753616 |
| PROVIDER: | scopus |
| PMCID: | PMC2526100 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 9" - "Export Date: 17 November 2011" - "CODEN: PNASA" - "Source: Scopus" |
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