Gene amplification in non-Hodgkins lymphoma Journal Article
| Authors: | Ben-Yehuda, D.; Houldsworth, J.; Parsa, N. Z.; Chaganti, R. S. K. |
| Article Title: | Gene amplification in non-Hodgkins lymphoma |
| Abstract: | Among 426 consecutively ascertained and karyotypically abnormal non-Hodgkin's lymphoma (NHL) tumours, cytological evidence for gene amplification in the form of homogeneously staining regions (HSRs) was encountered in nine cases of large cell diffuse lymphoma (LC-DL). The mean age of patients with HSRs was 62.9 years and four died within a year of diagnosis. To identify candidate gene(s) amplified in these tumours, we performed a Southern blot analysis of tumour DNA using probes for 23 known protooncogenes and the multidrug resistance gene, PGY1. Besides a two-fold implication of the BCL2 gene in two cases, no evidence for overt amplification of any of the genes assayed was found. To confirm DNA amplification in these specimens we performed the DNA in-gel renaturation assay. Evidence for presence of amplified DNA fragments was obtained in four of seven specimens. These results suggest amplification of a novel gene(s). To our knowledge, this is the first formal study of gene amplification in a large consecutively ascertained series of fresh lymphoma biopsies. |
| Keywords: | gene; amplification; receptor; follicular lymphoma; cytogenetic analysis; growth-factor; cell-lines; human homologs; proto-oncogene; homogeneously staining regions; non-hodgkins lymphoma; myc gene; clinical correlations; double minute chromosomes; human neuroblastomas |
| Journal Title: | British Journal of Haematology |
| Volume: | 86 |
| Issue: | 4 |
| ISSN: | 0007-1048 |
| Publisher: | John Wiley & Sons |
| Date Published: | 1994-04-01 |
| Start Page: | 792 |
| End Page: | 797 |
| Language: | English |
| ACCESSION: | WOS:A1994NG39400016 |
| DOI: | 10.1111/j.1365-2141.1994.tb04831.x |
| PROVIDER: | wos |
| PUBMED: | 7918074 |
| Notes: | Article -- Source: Wos |
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