High level of Nm23-H1 gene expression is associated with local colorectal cancer progression not with metastases Journal Article
| Authors: | Zeng, Z. S.; Hsu, S.; Zhang, Z. F.; Cohen, A. M.; Enker, W. E.; Turnbull, A. A.; Guillem, J. G. |
| Article Title: | High level of Nm23-H1 gene expression is associated with local colorectal cancer progression not with metastases |
| Abstract: | This study aimed to determine the expression of Nm23-H1 in colorectal cancer and liver metastases and to correlate Nm23-H1 expression with clinicopathological variables. Specimens from 59 primary colorectal cancers and five liver metastases were studied using Northern blot hybridisation. The mean +/- s.e. of tumour/normal (T/N) ratio of Nm23-H1 RNA expression was 4.3 +/- 0.4 (P<0.001) and 5.1 +/- 0.90 (P<0.01) for colorectal cancer and liver metasases respectively. No significant relationship was observed between the level of Nm23-H1 RNA and the patient's age, sex, tumour location, differentiation, presence of lymph node involvement or distant metastases. Nm23-H1 RNA level was 2.6 +/- 0.5 for tumour size less than 3.0 cm and 4.6 +/- 0.5 for those greater than or equal to 3.0 cm (P = 0.05). There appeared to be a trend between increasing relative Nm23-H1 RNA and bowel wall invasion, irrespective of metastatic status (T1 = 1.9 +/- 0.3, T2 = 4.1 +/- 0.6, T3 = 4.1 +/- 0.5 and T4 = 6.4 +/- 1.6). This difference was statistically significant when T1 was compared against greater than or equal to T2 lesions (P = 0.01). Western blot analysis reveals two Nm23H-1 bands (17.0 kDa and 18.5 kDa). In 16 colorectal patients, the T/N fold-increase in protein expression was 2.66 +/- 0.46 (P<0.001) and 2.40 +/- 0.32 (P<0.001) for the 17.0 and 18.5 kDa band respectively. Both Nm23-H1 RNA and protein levels in primary colorectal cancers do not appear to correlate with synchronous regional or distant metastases. Since Nm23-H1 RNA expression is associated with increasing tumour size and tumour local invasion, Nm23-H1 RNA expression may be associated with local disease progression. |
| Keywords: | protein expression; metastasis; drosophila; development; c-myc; nucleoside diphosphate kinase; lymph-node; human breast-cancer; messenger-rna; tumor-metastasis; reduced expression; good prognosis |
| Journal Title: | British Journal of Cancer |
| Volume: | 70 |
| Issue: | 5 |
| ISSN: | 0007-0920 |
| Publisher: | Nature Publishing Group |
| Date Published: | 1994-11-01 |
| Start Page: | 1025 |
| End Page: | 1030 |
| Language: | English |
| ACCESSION: | WOS:A1994PP75600044 |
| DOI: | 10.1038/bjc.1994.442 |
| PROVIDER: | wos |
| PMCID: | PMC2033541 |
| PUBMED: | 7947079 |
| Notes: | Source: Wos |
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