Association between polymorphisms in the prostate-specific antigen (PSA) promoter and release of PSA Journal Article


Authors: Sävblom, C.; Giwercman, A.; Malm, J.; Halldén, C.; Lundin, K.; Lilja, H.; Giwercman, Y.
Article Title: Association between polymorphisms in the prostate-specific antigen (PSA) promoter and release of PSA
Abstract: Variations in serum prostate-specific antigen (PSA) have been ascribed to A/G nucleotide polymorphisms located at -158 bp (rs266882) and -4643 bp (rs925013), relative to the transcription start site within the promoter of the PSA gene. PSA is also an androgen receptor target (AR) gene and polymorphisms in AR gene are known to affect AR function. Our objective was to compare the impact of these A/G polymorphisms separately or in combination with AR CAG micro satellite on regulation of PSA secretion into seminal plasma and blood in young men. Leukocyte DNA was extracted from 291 conscripts and genotyping performed with the Sequenom Mass Array System. PSA was measured with an immunofluorometric assay. Linear regression analysis was used to test the association of polymorphism frequencies with serum and seminal plasma levels of PSA. PSA gene polymorphisms at -158 bp or -4643 bp did not alone influence total PSA (tPSA) levels in seminal plasma or in blood. Homozygotes for the A-allele at -158 bp in combination with CAG > 22 had significantly higher serum levels of tPSA than subjects carrying the G-allele (p = 0.01). In conclusion, the PSA gene polymorphisms did not importantly influence the levels of tPSA in seminal plasma or in blood. tPSA in serum was influenced by interactions between PSA promoter variants and AR CAG polymorphism. © 2008 European Academy of Andrology.
Keywords: adult; promoter region; prostate specific antigen; protein blood level; allele; genotype; prostate-specific antigen; regulatory mechanism; antigen specificity; homozygote; protein secretion; androgen receptor; immunoassay; genetic polymorphism; dna polymorphism; seminal plasma; cag; polymorphism; fluorometry; linear regression analysis; transcription initiation site
Journal Title: International Journal of Andrology
Volume: 32
Issue: 5
ISSN: 0105-6263
Publisher: Wiley Blackwell  
Date Published: 2009-10-01
Start Page: 479
End Page: 485
Language: English
DOI: 10.1111/j.1365-2605.2008.00882.x
PROVIDER: scopus
PUBMED: 18336535
DOI/URL:
Notes: --- - "Cited By (since 1996): 1" - "Export Date: 30 November 2010" - "CODEN: IJAND" - "Source: Scopus"
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  1. Hans Gosta Lilja
    345 Lilja