Live imaging of leukocyte recruitment in a zebrafish model of chemical liver injury Journal Article


Authors: Stoddard, M.; Huang, C.; Enyedi, B.; Niethammer, P.
Article Title: Live imaging of leukocyte recruitment in a zebrafish model of chemical liver injury
Abstract: Studying early immune responses to organ damage in situ requires animal models amenable to intravital imaging. Here, we used transparent zebrafish larvae, a powerful animal model for innate immunity, to measure leukocyte recruitment to damaged livers. Bath application of metronidazole (Mtz) to fish expressing nitroreductase (NTR) under a liver-specific promoter damaged the organ within 24 hours causing oxidative stress, distorted liver morphology, accumulation of TUNEL-positive cells, and transcriptional upregulation of apoptotic and antioxidant genes. Inflammatory gene transcription in damaged hepatocytes was attenuated. In line with predominant apoptosis, macrophages were massively recruited into Mtz/NTR-damaged livers. By contrast, neutrophil infiltration was more variable and delayed, consistent with less abundant necrosis and an attenuated inflammatory capacity of damaged hepatocytes. © 2019, The Author(s).
Journal Title: Scientific Reports
Volume: 9
ISSN: 2045-2322
Publisher: Nature Publishing Group  
Date Published: 2019-01-10
Start Page: 28
Language: English
DOI: 10.1038/s41598-018-36771-9
PUBMED: 30631093
PROVIDER: scopus
PMCID: PMC6328554
DOI/URL:
Notes: Export Date: 1 February 2019 -- Source: Scopus
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  1. Cong   Huang
    4 Huang