Sensitive in vivo imaging of T cells using a membrane-bound Gaussia princeps luciferase Journal Article
| Authors: | Santos, E. B.; Yeh, R.; Lee, J.; Nikhamin, Y.; Punzalan, B.; Perle, K. L.; Larson, S. M.; Sadelain, M.; Brentjens, R. J. |
| Article Title: | Sensitive in vivo imaging of T cells using a membrane-bound Gaussia princeps luciferase |
| Abstract: | We developed a new approach to bioluminescent T cell imaging using a membrane-anchored form of the Gaussia luciferase (GLuc) enzyme, termed extGLuc, which we could stably express in both mouse and human primary T cells. In vitro, extGLuc<sup>+</sup> cells emitted significantly higher bioluminescent signal when compared to cells expressing GLuc, Renilla luciferase (RLuc) or membrane-anchored RLuc (extRLuc). In vivo, mouse extGLuc<sup>+</sup> T cells showed higher bioluminescent signal when compared to GLuc<sup>+</sup> and RLuc<sup>+</sup> T cells. Application of this imaging approach to human T cells genetically modified to express tumor-specific chimeric antigen receptors (CARs) enabled us to show in vivo CAR-mediated T cell accumulation in tumor, T cell persistence over time and concomitant imaging of T cells and tumor cells modified to express firefly luciferase. This sensitive imaging technology has application to many in vivo cell-based studies in a wide array of mouse models. © 2009 Nature America, Inc. All rights reserved. |
| Keywords: | signal transduction; controlled study; protein expression; unclassified drug; nonhuman; flow cytometry; sensitivity and specificity; t lymphocyte; t-lymphocytes; mouse; animals; mice; animal tissue; image analysis; luciferase; in vivo study; mice, scid; mice, inbred balb c; mice, inbred c57bl; chimera; dna modification; cell membrane; tumor cell; luciferases; bioluminescence; lymphocyte antigen receptor; firefly luciferase; gaussia princeps luciferase; renilla luciferin 2 monooxygenase; in vitro selection; beetles; gaussia princeps (t. scott, 1894); renilla luciferase |
| Journal Title: | Nature Medicine |
| Volume: | 15 |
| Issue: | 3 |
| ISSN: | 1078-8956 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2009-03-01 |
| Start Page: | 338 |
| End Page: | 344 |
| Language: | English |
| DOI: | 10.1038/nm.1930 |
| PUBMED: | 19219023 |
| PROVIDER: | scopus |
| PMCID: | PMC2837150 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 11" - "Export Date: 30 November 2010" - "CODEN: NAMEF" - "Source: Scopus" |
