A targeted chain-termination mutation in the mouse Apc gene results in multiple intestinal tumors Journal Article

   


Authors: Fodde, R.; Edelmann, W.; Yang, K.; Van Leeuwen, C.; Carlson, C.; Renault, B.; Breukel, C.; Alt, E.; Lipkin, M.; Khan, P. M.; Kucherlapati, R.
Article Title: A targeted chain-termination mutation in the mouse Apc gene results in multiple intestinal tumors
Abstract: Germ-line mutations in the human adenomatous polyposis coli (APC) gene result in familial adenomatous polyposis, an autosomal dominant disorder characterized by the early onset of multiple adenomatous polyps in the large bowel with a high likelihood of developing colorectal carcinomas. To understand the role of APC in intestinal tumor formation, we have introduced a chain-termination mutation in the 15th exon of the mouse Apc gene and employed it to modify the endogenous gene by homologous recombination in embryonic stem cells. Mice which are heterozygous for the Apc gene modification progressively develop intestinal tumors in a manner that is similar to that observed in patients with familial adenomatous polyposis and in mice which carry a mutation called multiple intestinal neoplasia (Min). Our results indicate that the Apc gene modification is a critical event in the initiation of intestinal tumor formation and results in an autosomal dominant predisposition toward development of spontaneous colonic and intestinal tumors in mice.
Keywords: gene mutation; mutation; nonhuman; colorectal cancer; animal cell; mouse; animal; mice; animal tissue; gene targeting; embryo; animalia; mice, transgenic; molecular sequence data; base sequence; dna primers; intestine tumor; intestinal neoplasms; colon carcinogenesis; genes, lethal; adenomatous polyposis coli; colon polyposis; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; animal model for human disease
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 91
Issue: 19
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 1994-09-13
Start Page: 8969
End Page: 8973
Language: English
DOI: 10.1073/pnas.91.19.8969
PROVIDER: scopus
PMCID: PMC44728
PUBMED: 8090754
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028567875&doi=10.1073%2fpnas.91.19.8969&partnerID=40&md5=195662baa4496e3a46f030783f1c6f69
Notes: Export Date: 14 January 2019 -- Article -- CODEN: PNASA C2 -- Source: Scopus
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  2. Kan Yang
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