Adipocyte-specific transcription factor ARF6 is a heterodimeric complex of two nuclear hormone receptors, PPARγ and RXRα Journal Article
| Authors: | Tontonoz, P.; Graves, R. A.; Budavari, A. I.; Erdjument-Bromage, H.; Lui, M.; Hu, E.; Tempst, P.; Spiegelman, B. M. |
| Article Title: | Adipocyte-specific transcription factor ARF6 is a heterodimeric complex of two nuclear hormone receptors, PPARγ and RXRα |
| Abstract: | Previously, we Identified a novel transcription factor, ARF6, as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. In order to identify the proteins which comprise the adipocyte ARF6 complex, we have purified this DNA binding activity from a cultured adipocyte cell line. We have developed a system for growth and differentiation of HIB-1B brown adlpocytes in suspension culture that facilitates the production of large quantities of adipocyte nuclear extract. ARF6 was purified from HIB-1B nuclear extract by a combination of conventional and sequence-specific DNA affinity chromotography. Chemical sequencing and mass spectral analysis of tryptlc peptldes derived from the purified polypeptides Identifies the ARF6 complex as a heterodlmer of the retlnold X receptor α (RXRα) and the murine peroxisome proliferator activated receptor γ (PPARγ). Of the known PPARγ isoforms, PPARγ Is the predominant form expressed In adipose tissue. These results suggest that PPARγ serves a unique function among PPAγ family members as an important regulator of adipocyte-specific gene expression. © 1994 Oxford University Press. |
| Keywords: | controlled study; dna-binding proteins; nonhuman; mass spectrometry; protein analysis; animal cell; mouse; animal; mice; gene expression; cell growth; cell line; protein binding; transcription factor; cell differentiation; peptide; transcription factors; liver; amino acid sequence; molecular sequence data; cell culture; protein purification; peptide fragments; brown fat; base sequence; dna sequence; hormone receptor; dna binding; cell nucleus receptor; retinoid; receptors, cytoplasmic and nuclear; oligodeoxyribonucleotides; cell extract; affinity chromatography; brown adipose tissue; adipocytes; receptors, retinoic acid; enhancer elements (genetics); priority journal; article; peroxisome proliferator; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; spectrum analysis, mass |
| Journal Title: | Nucleic Acids Research |
| Volume: | 22 |
| Issue: | 25 |
| ISSN: | 0305-1048 |
| Publisher: | Oxford University Press |
| Date Published: | 1994-12-25 |
| Start Page: | 5628 |
| End Page: | 5634 |
| Language: | English |
| DOI: | 10.1093/nar/22.25.5628 |
| PROVIDER: | scopus |
| PMCID: | PMC310126 |
| PUBMED: | 7838715 |
| DOI/URL: | |
| Notes: | Export Date: 14 January 2019 -- Article -- CODEN: NARHA C2 -- Source: Scopus |
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