BCL-6, a POZ/zinc-finger protein, is a sequence-specific transcriptional repressor Journal Article
| Authors: | Chang, C. C.; Ye, B. H.; Chaganti, R. S. K.; Dalla-Favera, R. |
| Article Title: | BCL-6, a POZ/zinc-finger protein, is a sequence-specific transcriptional repressor |
| Abstract: | Approximately 40% of diffuse large cell lymphoma are associated with chromosomal translocations that deregulate the expression of the BCL6 gene by juxtaposing heterologous promoters to the BCL-6 coding domain. The BCL6 gene encodes a 95-kDa protein containing six C-terminal zinc-finger motifs and an N-terminal POZ domain, suggesting that it may function as a transcription factor. By using a DNA sequence selected for its ability to hind recombinant BCL-6 in vitro, we show here that BCL-6 is present in DNA-binding complexes in nuclear extracts from various B-cell lines. In transient transfection experiments, BCL6 can repress transcription from promoters linked to its DNA target sequence and this activity is dependent upon specific DNA-binding and the presence of an intact N-terminal half of the protein. We demonstrate that this part of the BCL6 molecule contains an autonomous transrepressor domain and that two noncontiguous regions, including the POZ motif, mediate maximum transrepressive activity. These results indicate that the BCL-6 protein can function as a sequence-specific transcriptional repressor and have implications for the role of BCL6 in normal lymphoid development and lymphomagenesis. |
| Keywords: | dna-binding proteins; proto-oncogene proteins; animals; mice; gene expression; cell line; transcription, genetic; transfection; b-lymphocytes; transcription factors; molecular sequence data; amino terminal sequence; recombinant fusion proteins; immunoprecipitation; plasmid; chromosome translocation; base sequence; dna sequence; binding sites; thymidine kinase; cell nucleus; large cell lymphoma; luciferases; zinc finger protein; repressor proteins; genetic code; 3t3 cells; oligodeoxyribonucleotides; lymphoid cell; zinc fingers; repressor gene; promoter regions (genetics); proto-oncogene proteins c-bcl-6; humans; priority journal; article |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 93 |
| Issue: | 14 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1996-07-09 |
| Start Page: | 6947 |
| End Page: | 6952 |
| Language: | English |
| DOI: | 10.1073/pnas.93.14.6947 |
| PUBMED: | 8692924 |
| PROVIDER: | scopus |
| PMCID: | PMC38914 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
