A protein complex required for signal-sequence-specific sorting and translocation Journal Article
| Authors: | Wiedmann, B.; Sakai, H.; Davis, T. A.; Wiedmann, M. |
| Article Title: | A protein complex required for signal-sequence-specific sorting and translocation |
| Abstract: | We have purified a nascent-polypeptide-associated complex (NAC) which prevents short ribosome-associated nascent polypeptides from inappropriate interactions with proteins in the cytosol. NAC binds nascent-polypeptide domains emerging from ribosomes unless a signal peptide is fully exposed. Depletion of cytosolic proteins (including NAC) from ribosomes carrying nascent polypeptides allows the signal recognition particle (SRP) to crosslink to polypeptides irrespective of whether or not they contain signal peptides. In the absence of cytosol, proteins lacking signal peptides can be mistranslocated into the endoplasmic reticulum in vitro, albeit with low efficiency. Readdition of NAC restores the specificity of SRP and fidelity of translocation. © 1994 Nature Publishing Group. |
| Keywords: | unclassified drug; proteins; animal; protein targeting; cell protein; protein binding; endoplasmic reticulum; amino acid sequence; molecular sequence data; protein processing, post-translational; protein purification; signal peptide; protein transport; peptides; cattle; biological transport; cytosol; ribosome protein; ribosomes; polypeptide; cross linking; cross-linking reagents; protein sorting signals; signal recognition particle; human; priority journal; article; nascent polypeptide associated complex; translation, genetic |
| Journal Title: | Nature |
| Volume: | 370 |
| Issue: | 6489 |
| ISSN: | 0028-0836 |
| Publisher: | Nature Publishing Group |
| Date Published: | 1994-08-11 |
| Start Page: | 434 |
| End Page: | 440 |
| Language: | English |
| DOI: | 10.1038/370434a0 |
| PROVIDER: | scopus |
| PUBMED: | 8047162 |
| DOI/URL: | |
| Notes: | Source: Scopus |
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