Unregulated exposure of the ribosomal M-site caused by NAC depletion results in delivery of non-secretory polypeptides to the Sec61 complex Journal Article

  


Authors: Möller, I.; Beatrix, B.; Kreibich, G.; Sakai, H.; Lauring, B.; Wiedmann, M.
Article Title: Unregulated exposure of the ribosomal M-site caused by NAC depletion results in delivery of non-secretory polypeptides to the Sec61 complex
Abstract: Nascent polypeptide associated complex (NAC) interacts with nascent polypeptides emerging from ribosomes. Both signal recognition particle (SRP) and NAC work together to ensure specificity in co-translational targeting by competing for binding to the ribosomal membrane attachment site. While SRP selects signal-containing ribosomes for targeting, NAC prevents targeting of signal peptide-less nascent chains to the endoplasmic reticulum membrane. Here we show that the ribosome binding that occurs in NAC's absence delivers signal-less nascent chains to the Sec61 complex, underscoring the danger of unregulated exposure of the ribosomal M-site. Recently, the idea that NAC prevents ribosome binding has been challenged. By carefully examining the physiologic NAC concentration in a variety of tissues from different species we here demonstrate that the discrepancy resulted from subphysiologic NAC concentrations. Copyright (C) 1998 Federation of European Biochemical Societies.
Keywords: signal transduction; controlled study; unclassified drug; nonhuman; animals; animal tissue; protein binding; membrane proteins; transcription, genetic; cloning, molecular; recombinant fusion proteins; protein synthesis; signal peptide; recombinant proteins; organ specificity; protein secretion; protein biosynthesis; cattle; trans-activators; ribosomes; polypeptide; wheat germ; ribosome; molecular chaperones; cell-free system; microsomes; signal recognition particle; male; priority journal; article; reticulocyte lysate; nascent polypeptide associated complex; ribosomal membrane attachment site; sec61 complex; nascent polypeptide related complex; triticum
Journal Title: FEBS Letters
Volume: 441
Issue: 1
ISSN: 0014-5793
Publisher: Wiley Blackwell  
Date Published: 1998-12-11
Start Page: 1
End Page: 5
Language: English
DOI: 10.1016/s0014-5793(98)01440-9
PUBMED: 9877153
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0038984880&doi=10.1016%2fS0014-5793%2898%2901440-9&partnerID=40&md5=b432b52da9604fbe32af26a37de27a6a
Notes: Article -- Export Date: 12 December 2016 -- Source: Scopus
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MSK Authors
  1. Martin Wiedmann
    44 Wiedmann
  2. Birgitta M Beatrix
    4 Beatrix
  3. Ines Moeller
    4 Moeller
  4. Brett P Lauring
    2 Lauring
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