Newer options for treating drug-resistant (MDR+) cancer cells using photoradiation therapy Journal Article
| Authors: | Gulati, S. C.; Lemoli, R. M.; Igarashi, T.; Atzpodien, J. |
| Article Title: | Newer options for treating drug-resistant (MDR+) cancer cells using photoradiation therapy |
| Abstract: | Emergence of drug resistance with conventional cytotoxic therapy is a major challenge towards the curability of many cancers, especially in patients undergoing autologous BMT with ex-vivo purged hematopoietic support. We have explored the potential role of photoradiation therapy in purging hematopoietic stem cells of various hematological malignancies. Benzoporphyrin derivative, monoacid ring A (BPD-MA), dihematoporphyrin ether (DHE), and MC-540 were evaluated for the "ex-vivo" purging of residual tumor cells from autologous bone marrow (BM) grafts. BPD-MA and DHE photosensitizing activity was tested against two human large cell lymphoma cell lines and colony forming-unit leukemia (CFU-L) derived from patients with acute myelogenous leukemia (AML). In mixing experiments four log elimination of tumor cell lines was observed after 1 hr of incubation with BPD-MA or DHE followed by white light exposure. By comparison, using the same concentration of BPD-MA or DHE, the mean recovery of normal BM progenitors was 4-5.2% for granulocyte-macrophage colony forming unit (CFU-GM) and 5-9.8% for burst forming unit erythroid (BFUE). The T lymphoblastic leukemia cell line CEM and its vinblastine (VBL)-resistant subline CEM/VBL100. along with the acute promyelocyte leukemia cell line HL-60 and its vincristine (VCR)-resistant subline HL-60/VCR, were also tested. Our results demonstrated the preferential cytotoxicity of BPD-MA and DHE toward neoplastic cell lines and CFU-L from AML patients. In addition, DHE was slightly more effective in purging tumor cells expressing the p-170 glycoprotein. These results suggest that photoradiation would be useful for "ex-vivo" purging of malignant cells. Other methods to deal with decreasing drug resistance are also detailed. © 1994 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted. |
| Keywords: | controlled study; leukemia; acute granulocytic leukemia; human cell; vincristine; cancer cell culture; cytotoxicity; drug resistance; tumor cells, cultured; cancer cell; lymphoma; hematopoietic stem cells; bone marrow cell; hematopoietic stem cell; large cell lymphoma; bone marrow transplantation; t cell leukemia; cyclosporine; multidrug resistance; colony forming unit gm; drug-resistance; glycoprotein p; photosensitizing agent; bone marrow purging; mdr; phototherapy; burst forming unit e; photosensitization; human; priority journal; article; support, non-u.s. gov't; porphyrin derivative; antobodies; bepridil; merocyanine; photofrin ii |
| Journal Title: | Leukemia and Lymphoma |
| Volume: | 12 |
| Issue: | 5-6 |
| ISSN: | 1042-8194 |
| Publisher: | Taylor & Francis Group |
| Date Published: | 1994-02-01 |
| Start Page: | 427 |
| End Page: | 433 |
| Language: | English |
| DOI: | 10.3109/10428199409073784 |
| PROVIDER: | scopus |
| PUBMED: | 8180606 |
| DOI/URL: | |
| Notes: | Source: Scopus |
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