Ex vivo treatment of myeloma cells by 4‐HC, VP‐16, LAK cells and antibodies Journal Article

  


Authors: Gulati, S. C.; Shimazaki, C.; Lemoli, R. M.; Atzpodien, J.; Clarkson, B. D.
Article Title: Ex vivo treatment of myeloma cells by 4‐HC, VP‐16, LAK cells and antibodies
Abstract: To assess the usefulness of 4‐hydroperoxycyclophosphamide (4‐HC) and Etoposide (VP‐16) as a purging agent for myeloma cells in bone marrow ex‐vivo, myeloma cell lines (SK‐RCS‐1, RPMI‐8226), lymphoma cell line (SK‐DHL‐2) and normal bone marrow (BM) cells were treated at different concentrations of 4‐HC, VP‐16. In separate experiments, LAK cells or antibodies were also used to treat the above cell lines. Clonogenic tumor cells from all three cell lines could be reduced by more than 4 logs, when treated alone or as a mixture with irradiated normal bone marrow cells at a 4‐HC concentration of 60 μmol/l. Under similar conditions, approximately 1% of normal BM myeloid progenitor granulocyte‐macrophage colony forming cells (CFU‐GM) survived. The results with LAK cells and antibodies were also encouraging. These observations support the use of various purging methods for myeloma cells for autologous bone marrow transplantation. © Munksgaard 1989
Keywords: conference paper; comparative study; methodology; interleukin 2; multiple myeloma; etoposide; cyclophosphamide; cytotoxicity; drug effect; drug screening; pathology; tumor cells, cultured; monoclonal antibody; immunology; antibodies, monoclonal; cell culture; hematopoietic stem cells; drug derivative; cytotoxicity, immunologic; bone marrow cell; hematopoietic stem cell; clonogenic assay; colony-forming units assay; cell separation; myeloma; magnetism; magnetics; bone marrow purging; myeloma cell; tumor stem cell assay; killer cell; 4 hydroperoxycyclophosphamide; lymphokine activated killer cell; human; article; killer cells; purging; lak cells; 4‐hc; perfosfamide
Journal Title: European Journal of Haematology
Volume: 43
Issue: Suppl. 51
ISSN: 0902-4441
Publisher: John Wiley & Sons  
Date Published: 1989-12-01
Start Page: 164
End Page: 172
Language: English
DOI: 10.1111/j.1600-0609.1989.tb01511.x
PUBMED: 2627987
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024935106&doi=10.1111%2fj.1600-0609.1989.tb01511.x&partnerID=40&md5=d8a1e746e8f0d62055ab5663756a545b
Notes: Article -- Export Date: 14 April 2020 -- Source: Scopus
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MSK Authors
  1. Bayard Clarkson
    220 Clarkson
  2. Subhash C. Gulati
    129 Gulati
  3. Roberto M. Lemoli
    18 Lemoli
  4. Jens Atzodien
    24 Atzodien
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