Stimulation of vaccinia topoisomerase I by nucleoside triphosphates Journal Article
| Authors: | Sekiguchi, J.; Shuman, S. |
| Article Title: | Stimulation of vaccinia topoisomerase I by nucleoside triphosphates |
| Abstract: | The rate of relaxation of supercoiled DNA by purified vaccinia topoisomerase I is stimulated 20-fold by 5 mM ATP. A similar effect is elicited by GTP, CTP, UTP, dATP, and adenosine 5'-(β,γ-imido)triphosphate. ATP-mediated rate enhancement requires salt as a coactivator. ADP and inorganic pyrophosphate also stimulate relaxation 10-20-fold, whereas AMP and inorganic phosphate have little effect. A model for allosteric activation of topoisomerase by nucleotides is suggested. |
| Keywords: | nonhuman; enzyme activation; enzyme activity; kinetics; escherichia coli; vaccinia virus; binding sites; dna topoisomerase; dna topoisomerases, type i; dna denaturation; magnesium; vaccinia; dna conformation; nucleotides; dna supercoiling; dna, superhelical; nucleoside triphosphate; salts; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s. |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 269 |
| Issue: | 47 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 1994-11-25 |
| Start Page: | 29760 |
| End Page: | 29764 |
| Language: | English |
| PROVIDER: | scopus |
| PUBMED: | 7961968 |
| DOI/URL: | |
| Notes: | Export Date: 14 January 2019 -- Article -- Source: Scopus |
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