A poxvirus-like type IB topoisomerase family in bacteria Journal Article
| Authors: | Krogh, B. O.; Shuman, S. |
| Article Title: | A poxvirus-like type IB topoisomerase family in bacteria |
| Abstract: | We report that diverse species of bacteria encode a type IB DNA topoisomerase that resembles vaccinia virus topoisomerase. Deinococcus radiodurans topoisomerase IB (DraTopIB), an exemplary member of this family, relaxes supercoiled DNA in the absence of a divalent cation or ATP. DraTopIB has a compact size (346 aa) and is a monomer in solution. Mutational analysis shows that the active site of DraTopIB is composed of the same constellation of catalytic side chains as the vaccinia enzyme. Sequence comparisons and limited proteolysis suggest that their folds are conserved. These findings imply an intimate evolutionary relationship between the poxvirus and bacterial type IB enzymes, and they engender a scheme for the evolution of topoisomerase IB and tyrosine recombinases from a common ancestral strand transferase in the bacterial domain. Remarkably, bacteria that possess topoisomerase IB appear to lack DNA topoisomerase III. |
| Keywords: | controlled study; nonhuman; protein domain; protein degradation; protein binding; tyrosine; mutational analysis; bacteria (microorganisms); time factors; molecular cloning; cloning, molecular; bacterial proteins; dna; amino acid sequence; molecular sequence data; sequence homology, amino acid; enzyme analysis; dna viruses; recombinant proteins; vaccinia virus; mutagenesis, site-directed; protein structure, tertiary; binding sites; dna mutational analysis; catalysis; nucleic acid conformation; protein folding; sequence homology; protein family; enzyme structure; catalytic domain; centrifugation, density gradient; genetic conservation; amino acid motifs; dna topoisomerase; dna topoisomerases, type i; multigene family; enzyme active site; poxvirus; poxviridae; dna, superhelical; glycerol; recombinase; deinococcus radiodurans; deinococcus; transferase; virus enzyme; posibacteria; dna topoisomerases, type i, bacterial; priority journal; article; virus typing |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 99 |
| Issue: | 4 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 2002-02-19 |
| Start Page: | 1853 |
| End Page: | 1858 |
| Language: | English |
| DOI: | 10.1073/pnas.032613199 |
| PUBMED: | 11830640 |
| PROVIDER: | scopus |
| PMCID: | PMC122283 |
| DOI/URL: | |
| Notes: | Export Date: 14 November 2014 -- Source: Scopus |
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