Removal of Rab GTP-binding proteins from Golgi membranes by GDP dissociation inhibitor inhibits inter-cisternal transport in the Golgi stacks Journal Article
| Authors: | Elazar, Z.; Mayer, T.; Rothman, J. E. |
| Article Title: | Removal of Rab GTP-binding proteins from Golgi membranes by GDP dissociation inhibitor inhibits inter-cisternal transport in the Golgi stacks |
| Abstract: | Rab proteins are a family of Ras-like GTPases involved in intracellular membrane traffic. Rab GDI, a cytosolic protein which inhibits the dissociation of GDP from various Rab proteins, is required to maintain a pool of Rab proteins in the cytosol. We describe the purification of a cytosolic factor from bovine liver that inhibits intra-cisternal transport between the Golgi stacks. We identify this factor as a Rab GDI. Half-maximal inhibition of transport was observed in the presence of the same concentration of GDI that is required for removal of Rab proteins from the Golgi. |
| Keywords: | nonhuman; comparative study; animal; animal tissue; gtp-binding proteins; intracellular transport; animalia; monoclonal antibody; amino acid sequence; molecular sequence data; sequence homology, amino acid; protein purification; sequence alignment; protein transport; immunoblotting; cattle; sequence homology; protein family; guanine nucleotide binding protein; biological transport; cytosol; in vitro; golgi complex; cho cells; guanosine diphosphate; bovinae; inhibitor protein; cell-free system; golgi apparatus; bos taurus; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; rab3 gtp-binding proteins; hamsters; liver cytosol |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 269 |
| Issue: | 2 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 1994-01-14 |
| Start Page: | 794 |
| End Page: | 797 |
| Language: | English |
| PROVIDER: | scopus |
| PUBMED: | 8288626 |
| DOI/URL: | |
| Notes: | Export Date: 14 January 2019 -- Article -- Source: Scopus |
