A rab protein is required for the assembly of SNARE complexes in the docking of transport vesicles Journal Article
| Authors: | Søgaard, M.; Tani, K.; Ye, R. R.; Geromanos, S.; Tempst, P.; Kirchhausen, T.; Rothman, J. E.; Söllner, T. |
| Article Title: | A rab protein is required for the assembly of SNARE complexes in the docking of transport vesicles |
| Abstract: | Rab proteins are generally required for transport vesicle docking. We have exploited yeast secretion mutants to demonstrate that a rab protein is required for v-SNAREs and t-SNAREs to assemble. The absence of the rab protein in the docking complex suggests that, in a broad sense, rab proteins participate in a reaction catalyzing SNARE complex assembly. In so doing, rab proteins could help impart an additional layer of specificity to vesicle docking. This mechanism likely involves the Sec1 homolog Sly1, which we identified in isolated docking complexes. We also report the identification of a novel v-SNARE (Ykt6p) component of the yeast ER-Golgi docking complex that has a CAAX box and is predicted to be lipid anchored. The surprising finding that docking complexes can contain many distinct species of SNAREs (Sed5p, Bos1p, Sec22p, Ykt6p, and likely Bet1p, p28, and p14) suggests that multimeric interactions are features of the fusion machinery, and may also improve the fidelity of vesicle targeting. © 1994. |
| Keywords: | controlled study; nonhuman; complex formation; models, biological; protein assembly; nerve tissue proteins; gtp-binding proteins; membrane proteins; intracellular transport; endoplasmic reticulum; amino acid sequence; molecular sequence data; hybrid protein; saccharomyces cerevisiae; escherichia coli; carrier proteins; membrane protein; recombinant protein; base sequence; yeast; saccharomyces cerevisiae proteins; guanine nucleotide binding protein; biological transport; cell mutant; golgi complex; membrane vesicle; fungal proteins; vesicular transport proteins; intracellular membranes; membrane fusion; docking protein; qb-snare proteins; membrane receptor; golgi apparatus; r-snare proteins; protein isoprenylation; rab gtp-binding proteins; priority journal; article; n-ethylmaleimide-sensitive proteins; farnesol; spheroplast; munc18 proteins |
| Journal Title: | Cell |
| Volume: | 78 |
| Issue: | 6 |
| ISSN: | 0092-8674 |
| Publisher: | Cell Press |
| Date Published: | 1994-09-23 |
| Start Page: | 937 |
| End Page: | 948 |
| Language: | English |
| DOI: | 10.1016/0092-8674(94)90270-4 |
| PROVIDER: | scopus |
| PUBMED: | 7923363 |
| DOI/URL: | |
| Notes: | Export Date: 14 January 2019 -- Article -- Source: Scopus |
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