Tobacco smoking, occupation, and p53 nuclear overexpression in early stage bladder cancer Journal Article


Authors: Zhang, Z. F.; Sarkis, A. S.; Cordon-Cardo, C.; Dalbagni, G.; Melamed, J.; Aprikian, A.; Pollack, D.; Sheinfeld, J.; Herr, H. W.; Fair, W. R.; Reuter, V. E.; Begg, C.
Article Title: Tobacco smoking, occupation, and p53 nuclear overexpression in early stage bladder cancer
Abstract: Epidemiological studies show an increased risk of bladder cancer associated with tobacco smoking and occupational exposures. Certain carcinogens in tobacco and occupational exposures cause DNA damage and may produce specific mutations. TP53 is considered a common target for carcinogenic agents, and mutations of this gene are reported to be the most frequent nuclear abnormalities in human cancer. In order to investigate the relationship between tobacco smoking, occupations, and altered patterns of p53 expression, we have analyzed a group of 109 incident patients with superficial transitional cell carcinoma of the bladder. We assessed p53 nuclear overexpression by the use of anti-p53 antibody PAb1801 and immunohistochemistry, and identified 45 of 109 patients (41%) displaying p53-positive phenotype. We observed a significant association between the number of cigarettes smoked per day and p53 nuclear overexpression (p = 0.02). The odds ratios were 2.3 for those smoking 1-2 packs per day and 8.4 for smoking more than 2 packs per day. Similar estimates were obtained after Controlling for age, sex, and race. Elevated odds ratios were also observed for dye-/ink-related (odds ratio = 2.0; 95% CI, 0.4-9.4) and cooking-related occupations (1.8, 0.6-5.0), although those were not statistically significant. These data support the hypothesis that certain carcinogens derived from cigarette smoking and occupations may induce TP53 mutations, which in turn are involved in early steps of bladder carcinogenesis. © 1994, American Association for Cancer Research. All rights reserved.
Keywords: immunohistochemistry; adult; aged; major clinical study; cigarette smoking; cancer risk; neoplasm staging; phenotype; gene expression; odds ratio; risk factors; smoking; protein p53; tumor suppressor gene; bladder neoplasms; dye; tobacco; carcinoma, transitional cell; bladder carcinoma; transitional cell carcinoma; bladder carcinogenesis; cooking; occupational exposure; middle age; dyes; occupational cancer; ink; human; male; female; priority journal; article; support, u.s. gov't, p.h.s.; chemical industry; cookery
Journal Title: Cancer Epidemiology Biomarkers and Prevention
Volume: 3
Issue: 1
ISSN: 1055-9965
Publisher: American Association for Cancer Research  
Date Published: 1994-01-01
Start Page: 19
End Page: 24
Language: English
PROVIDER: scopus c2 - 8118380
PUBMED: 8118380
DOI/URL:
Notes: Source: Scopus