Salvianolic acid B inhibits ERK and p38 MAPK signaling in TGF-Β1-stimulated human hepatic stellate cell line (LX-2) via distinct pathways Journal Article


Authors: Xu, L.; Lu, Z.
Article Title: Salvianolic acid B inhibits ERK and p38 MAPK signaling in TGF-Β1-stimulated human hepatic stellate cell line (LX-2) via distinct pathways
Abstract: Salvianolic acid B (SA-B) is water-soluble component of Radix Salvia miltiorrhiza. The previous work indicated that SA-B can inhibit MAPK and Smad signaling in activated hepatic stellate cells (HSCs) to perform anti-fibrotic activity Lv et al. 2010. However, some studies have shown that there is cross-talk between MAPK and Smad in certain cell types. Thus, the anti-fibrotic action of SA-B may be through the cross-talk. In order to clarify the mechanism of SA-B further, we knocked down Smad in LX-2 cells (SRV4) via RNAi, and then added TGF-1, and PD98059 or SB203580 and SA-B. The levels of p-MEK and p-p38 were inhibited by SA-B in SRV4 independent of TGFΒ-1. The expression of Col I and -SMA in SRV4 could be reduced by SA-B independent TGFΒ-1. SB203580 had not significant effect on p-MEK in SRV4 stimulated by TGFΒ-1. The levels of p-MEK in SRV4 were not increased significantly after TGFΒ-1 stimulation. PD98059 had no effect on the levels of p-p38 in SRV4 irrespective of TGF-1. In conclusion, SA-B inhibits the synthesis of Col I in LX-2 cells independent of TGF-1 stimulation, and the anti-fibrotic effect of SA-B is due to direct inhibition of p38 signaling and inhibition the cross-talk of Smad to ERK signaling.
Journal Title: Evidence-Based Complementary and Alternative Medicine
Volume: 2012
ISSN: 1741-427X
Publisher: Hindawi Publishing Corporation  
Date Published: 2012-01-01
Start Page: 960128
Language: English
DOI: 10.1155/2012/960128
PROVIDER: scopus
PMCID: PMC3155803
PUBMED: 21860657
DOI/URL:
Notes: --- - "Export Date: 2 November 2011" - "Source: Scopus"
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MSK Authors
  1. Zhigang Lu
    11 Lu