Processing of DNA structures via DNA unwinding and branch migration by the S. cerevisiae Mph1 protein Journal Article
| Authors: | Zheng, X. F.; Prakash, R.; Saro, D.; Longerich, S.; Niu, H.; Sung, P. |
| Article Title: | Processing of DNA structures via DNA unwinding and branch migration by the S. cerevisiae Mph1 protein |
| Abstract: | The budding yeast Mph1 protein, the putative ortholog of human FANCM, possesses a 3' to 5' DNA helicase activity and is capable of disrupting the D-loop structure to suppress chromosome arm crossovers in mitotic homologous recombination. Similar to FANCM, genetic studies have implicated Mph1 in DNA replication fork repair. Consistent with this genetic finding, we show here that Mph1 is able to mediate replication fork reversal, and to process the Holliday junction via DNA branch migration. Moreover, Mph1 unwinds 3' and 5' DNA Flap structures that bear key features of the D-loop. These biochemical results not only provide validation for a role of Mph1 in the repair of damaged replication forks, but they also offer mechanistic insights as to its ability to efficiently disrupt the D-loop intermediate. © 2011 Elsevier B.V. |
| Keywords: | controlled study; unclassified drug; nonhuman; dna replication; dna damage; homologous recombination; dna repair; dead-box rna helicases; dna; protein processing; saccharomyces cerevisiae; dna structure; helicase; saccharomyces cerevisiae proteins; saccharomycetales; saccharomyces cerevisiae protein; dna helicases; dna denaturation; recombination; replication fork; branch migration; d-loop; fork regression; mph1; protein mutator phenotype 1; holliday junction; dna, cruciform |
| Journal Title: | DNA Repair |
| Volume: | 10 |
| Issue: | 10 |
| ISSN: | 1568-7864 |
| Publisher: | Elsevier Inc. |
| Date Published: | 2011-10-10 |
| Start Page: | 1034 |
| End Page: | 1043 |
| Language: | English |
| DOI: | 10.1016/j.dnarep.2011.08.002 |
| PROVIDER: | scopus |
| PMCID: | PMC3185188 |
| PUBMED: | 21880555 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 2 November 2011" - "CODEN: DRNEA" - "Source: Scopus" |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work