Homologous recombination assay for interstrand cross-link repair Journal Article

   


Authors: Nakanishi, K.; Cavallo, F.; Brunet, E.; Jasin, M.
Article Title: Homologous recombination assay for interstrand cross-link repair
Title Series: Methods in Molecular Biology
Abstract: DNA interstrand cross-links (ICLs) covalently link both strands of the DNA duplex, impeding cellular processes like DNA replication. Homologous recombination (HR) is considered to be a major pathway for the repair of ICLs in mammalian cells as mutants for HR components are highly sensitive to DNA-damaging agents that cause ICLs. This chapter describes GFP assays to measure HR following site-specific ICL formation with psoralen through DNA triplex technology. This approach can be used to determine the genetic requirements for ICL-induced HR in relation to those involved in HR repair of other DNA lesions such as double-strand breaks.
Keywords: genetics; flow cytometry; dna repair; cell line, tumor; genetic recombination; recombination, genetic; tumor cell line; plasmid; plasmids; oligonucleotide; oligonucleotides
Journal Title: Methods in Molecular Biology
Volume: 745
ISSN: 1064-3745
Publisher: Humana Press Inc  
Date Published: 2011-01-01
Start Page: 283
End Page: 291
Language: English
DOI: 10.1007/978-1-61779-129-1_16
PUBMED: 21660700
PROVIDER: scopus
PMCID: PMC3261721
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-80053333958&partnerID=40&md5=0d217e478738c55d5e1ab3f0c054b560
Notes: Chapter 16 in "DNA Recombination: Methods and Protocols" (ISBN: 978-1-61779-128-4) --- Export Date: 2 November 2011 - Source: Scopus
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MSK Authors
  1. Koji Nakanishi
    8 Nakanishi
  2. Maria Jasin
    249 Jasin
  3. Francesca Cavallo
    9 Cavallo
  4. Erika Simone Suzanne Brunet
    6 Brunet
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