Structure of the FANCI-FANCD2 complex: Insights into the Fanconi anemia DNA repair pathway Journal Article
| Authors: | Joo, W.; Xu, G.; Persky, N. S.; Smogorzewska, A.; Rudge, D. G.; Buzovetsky, O.; Elledge, S. J.; Pavletich, N. P. |
| Article Title: | Structure of the FANCI-FANCD2 complex: Insights into the Fanconi anemia DNA repair pathway |
| Abstract: | Fanconi anemia is a cancer predisposition syndrome caused by defects in the repair of DNA interstrand cross-links (ICLs). Central to this pathway is the Fanconi anemia I - Fanconi anemia D2 (FANCI-FANCD2) (ID) complex, which is activated by DNA damage - induced phosphorylation and monoubiquitination. The 3.4 angstrom crystal structure of the ∼300 kilodalton ID complex reveals that monoubiquitination and regulatory phosphorylation sites map to the I-D interface, suggesting that they occur on monomeric proteins or an opened-up complex and that they may serve to stabilize I-D heterodimerization. The 7.8 angstrom electron-density map of FANCI-DNA crystals and in vitro data show that each protein has binding sites for both single- and double-stranded DNA, suggesting that the ID complex recognizes DNA structures that result from the encounter of replication forks with an ICL. |
| Journal Title: | Science |
| Volume: | 333 |
| Issue: | 6040 |
| ISSN: | 0036-8075 |
| Publisher: | American Association for the Advancement of Science |
| Date Published: | 2011-07-15 |
| Start Page: | 312 |
| End Page: | 316 |
| Language: | English |
| DOI: | 10.1126/science.1205805 |
| PROVIDER: | scopus |
| PUBMED: | 21764741 |
| PMCID: | PMC3310437 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 17 August 2011" - "CODEN: SCIEA" - "Source: Scopus" |
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